A sequence-specific DNA-binding protein that activates fushi tarazu segmentation gene expression

Hitoshi Ueda, Sandra Sonoda, J. Lesley Brown, Matthew P. Scott, Carl Wu

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

The Drosophila segmentation gene fushi tarazu (ftz) is expressed at the cellular blastoderm stage in a pattern of seven transverse stripes; the stripes lie out of register with the segmental primordia, spanning alternate segmental boundaries. The zebra element, a 740-bp DNA sequence upstream of the ftz translational start, directs striped expression of lacZ when introduced into the fly genome. We have purified to homogeneity a sequence-specific DNA-binding factor, FTZ-F1, that binds to two sites located within the zebra element and to two sites within the ftz protein-coding sequence. FTZ-F1 DNA-binding activity is first detected in extracts of 1.5- to 4-hr embryos, coincident with the time of ftz expression in stripes; the activity then diminishes before reappearing during late embryo, larval, and adult stages. When one of the FTZ-F1-binding sequences in the zebra element is mutated by 2- or 4-base substitutions, the binding to FTZ-F1 is disrupted in vitro, and the intensity of lacZ expression is reduced in transformed embryos, especially in stripes 1, 2, 3, and 6. The results suggest that FTZ-F1 is a transcriptional activator necessary for the proper expression of the ftz gene.

Original languageEnglish
Pages (from-to)624-635
Number of pages12
JournalGenes and Development
Volume4
Issue number4
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Equidae
DNA-Binding Proteins
Embryonic Structures
Gene Expression
Fushi Tarazu Transcription Factors
Blastoderm
Diptera
Genes
Drosophila
Genome
DNA

Keywords

  • Ftz gene
  • FTZ-F1
  • Fushi tarazu

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

A sequence-specific DNA-binding protein that activates fushi tarazu segmentation gene expression. / Ueda, Hitoshi; Sonoda, Sandra; Lesley Brown, J.; Scott, Matthew P.; Wu, Carl.

In: Genes and Development, Vol. 4, No. 4, 1990, p. 624-635.

Research output: Contribution to journalArticle

Ueda, H, Sonoda, S, Lesley Brown, J, Scott, MP & Wu, C 1990, 'A sequence-specific DNA-binding protein that activates fushi tarazu segmentation gene expression', Genes and Development, vol. 4, no. 4, pp. 624-635.
Ueda, Hitoshi ; Sonoda, Sandra ; Lesley Brown, J. ; Scott, Matthew P. ; Wu, Carl. / A sequence-specific DNA-binding protein that activates fushi tarazu segmentation gene expression. In: Genes and Development. 1990 ; Vol. 4, No. 4. pp. 624-635.
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