A sensitive spectrofluorimetric determination of human serum albumin with chrome azurol S

Yutaka Saito, Yukiko Inden-Okazaki, Shoko Wada-Yano, Atsuko Kanetsuna, Kei Ichi Miyazaki, Masaki Mifune, Yoshimasa Tanaka, Hideki Okuda

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A simple and sensitive method for assay of human serum albumin (5-80 μg) is based on binding of chrome azurol S by the albumin and determination of the bound dye spectrofluorimetrically. The method is sufficiently sensitive for application to spinal fluid (20-100 μl).

Original languageEnglish
Pages (from-to)337-339
Number of pages3
JournalAnalytica Chimica Acta
Volume178
Issue numberC
DOIs
Publication statusPublished - 1985

Fingerprint

Serum Albumin
serum
Albumins
Assays
Coloring Agents
Fluids
dye
assay
fluid
chrome azurol S
method

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Saito, Y., Inden-Okazaki, Y., Wada-Yano, S., Kanetsuna, A., Miyazaki, K. I., Mifune, M., ... Okuda, H. (1985). A sensitive spectrofluorimetric determination of human serum albumin with chrome azurol S. Analytica Chimica Acta, 178(C), 337-339. https://doi.org/10.1016/S0003-2670(00)86286-8

A sensitive spectrofluorimetric determination of human serum albumin with chrome azurol S. / Saito, Yutaka; Inden-Okazaki, Yukiko; Wada-Yano, Shoko; Kanetsuna, Atsuko; Miyazaki, Kei Ichi; Mifune, Masaki; Tanaka, Yoshimasa; Okuda, Hideki.

In: Analytica Chimica Acta, Vol. 178, No. C, 1985, p. 337-339.

Research output: Contribution to journalArticle

Saito, Y, Inden-Okazaki, Y, Wada-Yano, S, Kanetsuna, A, Miyazaki, KI, Mifune, M, Tanaka, Y & Okuda, H 1985, 'A sensitive spectrofluorimetric determination of human serum albumin with chrome azurol S', Analytica Chimica Acta, vol. 178, no. C, pp. 337-339. https://doi.org/10.1016/S0003-2670(00)86286-8
Saito, Yutaka ; Inden-Okazaki, Yukiko ; Wada-Yano, Shoko ; Kanetsuna, Atsuko ; Miyazaki, Kei Ichi ; Mifune, Masaki ; Tanaka, Yoshimasa ; Okuda, Hideki. / A sensitive spectrofluorimetric determination of human serum albumin with chrome azurol S. In: Analytica Chimica Acta. 1985 ; Vol. 178, No. C. pp. 337-339.
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