A sensitive enzyme immunoassay for human basic fibroblast growth factor

Hiroyuki Watanabe, Akira Hori, Masaharu Seno, Yoshio Kozai, Koichi Igarashi, Yuzo Ichimori, Koichi Kondo

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number1
DOIs
Publication statusPublished - Feb 28 1991
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Immunoenzyme Techniques
Assays
Enzymes
Fibroblast Growth Factor 1
Immunoglobulin Fab Fragments
Horseradish Peroxidase
Bioactivity
Tumors
Tumor Cell Line
Monoclonal Antibodies
Cells
Limit of Detection
Healthy Volunteers
Acids
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A sensitive enzyme immunoassay for human basic fibroblast growth factor. / Watanabe, Hiroyuki; Hori, Akira; Seno, Masaharu; Kozai, Yoshio; Igarashi, Koichi; Ichimori, Yuzo; Kondo, Koichi.

In: Biochemical and Biophysical Research Communications, Vol. 175, No. 1, 28.02.1991, p. 229-235.

Research output: Contribution to journalArticle

Watanabe, Hiroyuki ; Hori, Akira ; Seno, Masaharu ; Kozai, Yoshio ; Igarashi, Koichi ; Ichimori, Yuzo ; Kondo, Koichi. / A sensitive enzyme immunoassay for human basic fibroblast growth factor. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 175, No. 1. pp. 229-235.
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