Abstract
A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.
| Original language | English |
|---|---|
| Pages (from-to) | 229-235 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 175 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 28 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A sensitive enzyme immunoassay for human basic fibroblast growth factor. / Watanabe, Hiroyuki; Hori, Akira; Seno, Masaharu; Kozai, Yoshio; Igarashi, Koichi; Ichimori, Yuzo; Kondo, Koichi.
In: Biochemical and Biophysical Research Communications, Vol. 175, No. 1, 28.02.1991, p. 229-235.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A sensitive enzyme immunoassay for human basic fibroblast growth factor
AU - Watanabe, Hiroyuki
AU - Hori, Akira
AU - Seno, Masaharu
AU - Kozai, Yoshio
AU - Igarashi, Koichi
AU - Ichimori, Yuzo
AU - Kondo, Koichi
PY - 1991/2/28
Y1 - 1991/2/28
N2 - A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.
AB - A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.
UR - http://www.scopus.com/inward/record.url?scp=0026081021&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026081021&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)81224-0
DO - 10.1016/S0006-291X(05)81224-0
M3 - Article
VL - 175
SP - 229
EP - 235
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -