A sensitive enzyme immunoassay for human basic fibroblast growth factor

Hiroyuki Watanabe; Akira Hori; Masaharu Seno; Yoshio Kozai; Koichi Igarashi; Yuzo Ichimori; Koichi Kondo

doi:10.1016/S0006-291X(05)81224-0

A sensitive enzyme immunoassay for human basic fibroblast growth factor

Hiroyuki Watanabe, Akira Hori, Masaharu Seno, Yoshio Kozai, Koichi Igarashi, Yuzo Ichimori, Koichi Kondo

Research output: Contribution to journal › Article › peer-review

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Abstract

A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.

Original language	English
Pages (from-to)	229-235
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	175
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(05)81224-0
Publication status	Published - Feb 28 1991
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.",

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AU - Watanabe, Hiroyuki

AU - Hori, Akira

AU - Seno, Masaharu

AU - Kozai, Yoshio

AU - Igarashi, Koichi

AU - Ichimori, Yuzo

AU - Kondo, Koichi

PY - 1991/2/28

Y1 - 1991/2/28

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AB - A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neiter human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this assay system was 1 pg/well. Using this assay, some tumor cell lines were revealed to produce a higher level of bFGF than a normal one. Serum samples from normal volunteers were also assayed, and immuno-reactive HbFGF could be detected in 16 out of 57 samples at range 30∼206 pg/ml.

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A sensitive enzyme immunoassay for human basic fibroblast growth factor

Abstract

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