A sensitive assay for mutagenic activity of N-nitrosamines and its use for detection of modulators of the mutagenicity.

T. Negishi, Y. Ohara, H. Hayatsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

By carrying out the pre-incubation of bacteria with N-nitrosamines under acidic conditions, the mutagenicity of N-nitrosodimethylamine on Salmonella TA100 and E. coli WP2 try, hcr was detected with increased sensitivity, compared to usual assays in neutral buffers. Using this modified assay system, compounds have been screened for their modulating effects on N-nitrosamine-mediated mutagenesis and the following were found to be inhibitors: cysteine, cysteamine, bisulfite, n-propylamine, n-butylamine, n-hexylamine, n-octylamine, n-nonylamine, serotonine, tryptamine, epinephrine, norepinephrine and oleic acid. The mechanisms of inhibition are discussed.

Original languageEnglish
Pages (from-to)685-694
Number of pages10
JournalIARC scientific publications
Issue number41
Publication statusPublished - 1982
Externally publishedYes

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Nitrosamines
Propylamines
Cysteamine
Dimethylnitrosamine
Oleic Acid
Salmonella
Mutagenesis
Epinephrine
Cysteine
Norepinephrine
Buffers
Escherichia coli
Bacteria
n-butylamine
hexylamine
octylamine
tryptamine
hydrogen sulfite

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A sensitive assay for mutagenic activity of N-nitrosamines and its use for detection of modulators of the mutagenicity. / Negishi, T.; Ohara, Y.; Hayatsu, H.

In: IARC scientific publications, No. 41, 1982, p. 685-694.

Research output: Contribution to journalArticle

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