A semi-dominant mutation in the ribosomal protein L10 gene suppresses the dwarf phenotype of the acl5 mutant in Arabidopsis thaliana

Akihiro Imai, Mio Komura, Eri Kawano, Yoshitaka Kuwashiro, Taku Takahashi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Summary Disruption of the Arabidopsis thaliana ACAULIS5 (ACL5) gene, which has recently been shown to encode thermospermine synthase, results in a severe dwarf phenotype. A previous study showed that sac51-d, a dominant suppressor mutant of acl5-1, has a premature termination codon in an upstream open reading frame (ORF) of SAC51, which encodes a putative transcription factor, and suggested the involvement of upstream ORF-mediated translational control in ACL5-dependent stem elongation. Here we report the identification of a gene responsible for sac52-d, another semi-dominant suppressor mutant of acl5-1. SAC52 encodes ribosomal protein L10 (RPL10A), which is highly conserved among eukaryotes and implicated in translational regulation. Transformation of acl5-1 mutants with a genomic fragment containing the sac52-d allele rescued the dwarf phenotype of acl5-1. GUS reporter activity under the control of a SAC51 promoter with its upstream ORF was higher in acl5-1 sac52-d than in acl5-1, suggesting that suppression of the acl5-1 phenotype by sac52-d is attributable, in part, to enhanced translation of certain transcripts including SAC51. We also found that a T-DNA insertion allele of SAC52/RPL10A causes lethality in the female gametophyte.

Original languageEnglish
Pages (from-to)881-890
Number of pages10
JournalPlant Journal
Volume56
Issue number6
DOIs
Publication statusPublished - Dec 2008

Fingerprint

ribosomal proteins
Arabidopsis
Open Reading Frames
open reading frames
Arabidopsis thaliana
Phenotype
mutation
phenotype
mutants
Mutation
Alleles
Genes
alleles
genes
stop codon
Nonsense Codon
stem elongation
Eukaryota
translation (genetics)
eukaryotic cells

Keywords

  • ACL5
  • Arabidopsis
  • Dwarf
  • Polyamine
  • Ribosomal protein
  • Translation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

A semi-dominant mutation in the ribosomal protein L10 gene suppresses the dwarf phenotype of the acl5 mutant in Arabidopsis thaliana. / Imai, Akihiro; Komura, Mio; Kawano, Eri; Kuwashiro, Yoshitaka; Takahashi, Taku.

In: Plant Journal, Vol. 56, No. 6, 12.2008, p. 881-890.

Research output: Contribution to journalArticle

Imai, Akihiro ; Komura, Mio ; Kawano, Eri ; Kuwashiro, Yoshitaka ; Takahashi, Taku. / A semi-dominant mutation in the ribosomal protein L10 gene suppresses the dwarf phenotype of the acl5 mutant in Arabidopsis thaliana. In: Plant Journal. 2008 ; Vol. 56, No. 6. pp. 881-890.
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