A Root-specific O-Methyltransferase Gene Expressed in Salt-tolerant Barley

Manabu Sugimoto, Yoshihiro Okada, Kazuhiro Sato, Kazutoshi Ito, Kazuyoshi Takeda

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.

    Original languageEnglish
    Pages (from-to)966-972
    Number of pages7
    JournalBioscience, Biotechnology and Biochemistry
    Volume67
    Issue number5
    DOIs
    Publication statusPublished - Jan 1 2003

    Keywords

    • Acetic acid bacteria
    • Hordeum vulgare L.
    • O-methyltransferase
    • Root
    • Salt stress
    • Subtraction hybridization

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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