A Root-specific O-Methyltransferase Gene Expressed in Salt-tolerant Barley

Manabu Sugimoto, Yoshihiro Okada, Kazuhiro Sato, Kazutoshi Ito, Kazuyoshi Takeda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.

Original languageEnglish
Pages (from-to)966-972
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number5
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Acetic acid bacteria
  • Hordeum vulgare L.
  • O-methyltransferase
  • Root
  • Salt stress
  • Subtraction hybridization

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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