A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line

Haruaki Nishimaki; Kenji Kasai; Ken Ichi Kozaki; Tomohiro Takeo; Hiroshi Ikeda; Shinsuke Saga; Masakazu Nitta; Gen Itoh

doi:10.1016/j.bbrc.2003.12.097

A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line

Haruaki Nishimaki, Kenji Kasai, Ken Ichi Kozaki, Tomohiro Takeo, Hiroshi Ikeda, Shinsuke Saga, Masakazu Nitta, Gen Itoh

Dental School

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Sonic hedgehog (Shh) is a secreted morphogen crucial for appropriate cellular proliferation during mammalian development. The activated Shh signaling is known to predispose to human tumors such as medulloblastoma and basal cell carcinoma, while a role of Shh signaling in the other common tumors is still controversial. Here we showed the overexpression of Shh in five cell lines among 14 human oral squamous cell carcinoma (OSCC) cell lines. One of the Shh-expressing OSCC cell lines HSQ-89 showed the inhibition of G1/S transition and apoptotic cell death by treatment with Cyclopamine, a steroidal alkaloid that blocks the intracellular Shh signaling. Furthermore, we found that treatment with Y-27632, a specific inhibitor of Rho-associated kinase, mimicked the effect of Cyclopamine on the cell cycle progression of HSQ-89. Our study revealed the involvement of activated Shh signaling in the cellular proliferation of OSCC cells, indicating Shh signaling might be a good therapeutic target for OSCC.

Original language	English
Pages (from-to)	313-320
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	314
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2003.12.097
Publication status	Published - Feb 6 2004

Keywords

Apoptosis
Cell cycle
Cyclopamine
Oral cancer
Rho-associated kinase
Smoothened
Sonic hedgehog
Squamous cell carcinoma
Y-27632

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2003.12.097

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title = "A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line",

abstract = "Sonic hedgehog (Shh) is a secreted morphogen crucial for appropriate cellular proliferation during mammalian development. The activated Shh signaling is known to predispose to human tumors such as medulloblastoma and basal cell carcinoma, while a role of Shh signaling in the other common tumors is still controversial. Here we showed the overexpression of Shh in five cell lines among 14 human oral squamous cell carcinoma (OSCC) cell lines. One of the Shh-expressing OSCC cell lines HSQ-89 showed the inhibition of G1/S transition and apoptotic cell death by treatment with Cyclopamine, a steroidal alkaloid that blocks the intracellular Shh signaling. Furthermore, we found that treatment with Y-27632, a specific inhibitor of Rho-associated kinase, mimicked the effect of Cyclopamine on the cell cycle progression of HSQ-89. Our study revealed the involvement of activated Shh signaling in the cellular proliferation of OSCC cells, indicating Shh signaling might be a good therapeutic target for OSCC.",

keywords = "Apoptosis, Cell cycle, Cyclopamine, Oral cancer, Rho-associated kinase, Smoothened, Sonic hedgehog, Squamous cell carcinoma, Y-27632",

author = "Haruaki Nishimaki and Kenji Kasai and Kozaki, {Ken Ichi} and Tomohiro Takeo and Hiroshi Ikeda and Shinsuke Saga and Masakazu Nitta and Gen Itoh",

note = "Funding Information: We thank Dr. Masahide Takahashi for critical reading and comments. We also thank Mr. Makoto Naruse for his technical support on FACS analysis. This work was supported in part by a grant to K. Kasai from Daiko Foundation.",

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AU - Takeo, Tomohiro

AU - Ikeda, Hiroshi

AU - Saga, Shinsuke

AU - Nitta, Masakazu

AU - Itoh, Gen

N1 - Funding Information: We thank Dr. Masahide Takahashi for critical reading and comments. We also thank Mr. Makoto Naruse for his technical support on FACS analysis. This work was supported in part by a grant to K. Kasai from Daiko Foundation.

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A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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