A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line

Haruaki Nishimaki, Kenji Kasai, Ken Ichi Kozaki, Tomohiro Takeo, Hiroshi Ikeda, Shinsuke Saga, Masakazu Nitta, Gen Itoh

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Sonic hedgehog (Shh) is a secreted morphogen crucial for appropriate cellular proliferation during mammalian development. The activated Shh signaling is known to predispose to human tumors such as medulloblastoma and basal cell carcinoma, while a role of Shh signaling in the other common tumors is still controversial. Here we showed the overexpression of Shh in five cell lines among 14 human oral squamous cell carcinoma (OSCC) cell lines. One of the Shh-expressing OSCC cell lines HSQ-89 showed the inhibition of G1/S transition and apoptotic cell death by treatment with Cyclopamine, a steroidal alkaloid that blocks the intracellular Shh signaling. Furthermore, we found that treatment with Y-27632, a specific inhibitor of Rho-associated kinase, mimicked the effect of Cyclopamine on the cell cycle progression of HSQ-89. Our study revealed the involvement of activated Shh signaling in the cellular proliferation of OSCC cells, indicating Shh signaling might be a good therapeutic target for OSCC.

Original languageEnglish
Pages (from-to)313-320
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume314
Issue number2
DOIs
Publication statusPublished - Feb 6 2004
Externally publishedYes

Fingerprint

Squamous Cell Carcinoma
Cells
Cell Proliferation
Cell Line
Tumors
rho-Associated Kinases
Medulloblastoma
Basal Cell Carcinoma
Alkaloids
Cell death
Neoplasms
Cell Cycle
Cell Death
Epithelial Cells
cyclopamine
Therapeutics

Keywords

  • Apoptosis
  • Cell cycle
  • Cyclopamine
  • Oral cancer
  • Rho-associated kinase
  • Smoothened
  • Sonic hedgehog
  • Squamous cell carcinoma
  • Y-27632

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line. / Nishimaki, Haruaki; Kasai, Kenji; Kozaki, Ken Ichi; Takeo, Tomohiro; Ikeda, Hiroshi; Saga, Shinsuke; Nitta, Masakazu; Itoh, Gen.

In: Biochemical and Biophysical Research Communications, Vol. 314, No. 2, 06.02.2004, p. 313-320.

Research output: Contribution to journalArticle

Nishimaki, Haruaki ; Kasai, Kenji ; Kozaki, Ken Ichi ; Takeo, Tomohiro ; Ikeda, Hiroshi ; Saga, Shinsuke ; Nitta, Masakazu ; Itoh, Gen. / A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 314, No. 2. pp. 313-320.
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