A role for phosphorylation in the proteolytic processing of the human NF-KB1 precursor

Koutarou Fujimoto, Hideyo Yasuda, Yoshihisa Sato, Ken-ichi Yamamoto

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A precursor, p105, for one of the subunits (p50) of the NF-κB transcription factor, plays an important role in inducible expression of diverse cellular genes, p105 also functions as a cytoplasmic inhibitor for NF-κB, and the proteolytic processing of its inhibitory C-terminal region is required for generation of active NF-κB. Here, it is reported that the human p105 C-terminal region is phosphorylated in vivo on Ser894 and Ser908, which are potential phosphorylation sites in vitro for proline-directed serine/threonine kinases such as cyclin-dependent kinase. Furthermore, the mutation of these in vivo phosphorylation sites retards p105 processing in vivo, suggesting that p105 processing is regulated in a phosphorylation-dependent manner.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalGene
Volume165
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Phosphorylation
Cyclin-Dependent Kinases
Protein-Serine-Threonine Kinases
Proline
Transcription Factors
Mutation
Genes
In Vitro Techniques
3'-(1-butylphosphoryl)adenosine

Keywords

  • inflammation
  • p105
  • proteasome
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

Cite this

A role for phosphorylation in the proteolytic processing of the human NF-KB1 precursor. / Fujimoto, Koutarou; Yasuda, Hideyo; Sato, Yoshihisa; Yamamoto, Ken-ichi.

In: Gene, Vol. 165, No. 2, 1995, p. 183-189.

Research output: Contribution to journalArticle

Fujimoto, Koutarou ; Yasuda, Hideyo ; Sato, Yoshihisa ; Yamamoto, Ken-ichi. / A role for phosphorylation in the proteolytic processing of the human NF-KB1 precursor. In: Gene. 1995 ; Vol. 165, No. 2. pp. 183-189.
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