TY - JOUR
T1 - A role for phosphorylation in the proteolytic processing of the human NF-KB1 precursor
AU - Fujimoto, Koutarou
AU - Yasuda, Hideyo
AU - Sato, Yoshihisa
AU - Yamamoto, Ken ichi
N1 - Funding Information:
We thank A. Israel for human p105 cDNA, C.A. Rosen for the p65 expression plasmid and E. Nishida for purified Xenopus MAPK (Erk 2). This work was supported in part by Grants-in Aid from the Ministry of Education, Science and Culture of Japan and the Hokkoku Cancer Research Foundation.
PY - 1995
Y1 - 1995
N2 - A precursor, p105, for one of the subunits (p50) of the NF-κB transcription factor, plays an important role in inducible expression of diverse cellular genes, p105 also functions as a cytoplasmic inhibitor for NF-κB, and the proteolytic processing of its inhibitory C-terminal region is required for generation of active NF-κB. Here, it is reported that the human p105 C-terminal region is phosphorylated in vivo on Ser894 and Ser908, which are potential phosphorylation sites in vitro for proline-directed serine/threonine kinases such as cyclin-dependent kinase. Furthermore, the mutation of these in vivo phosphorylation sites retards p105 processing in vivo, suggesting that p105 processing is regulated in a phosphorylation-dependent manner.
AB - A precursor, p105, for one of the subunits (p50) of the NF-κB transcription factor, plays an important role in inducible expression of diverse cellular genes, p105 also functions as a cytoplasmic inhibitor for NF-κB, and the proteolytic processing of its inhibitory C-terminal region is required for generation of active NF-κB. Here, it is reported that the human p105 C-terminal region is phosphorylated in vivo on Ser894 and Ser908, which are potential phosphorylation sites in vitro for proline-directed serine/threonine kinases such as cyclin-dependent kinase. Furthermore, the mutation of these in vivo phosphorylation sites retards p105 processing in vivo, suggesting that p105 processing is regulated in a phosphorylation-dependent manner.
KW - Transcription factor
KW - inflammation
KW - p105
KW - proteasome
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U2 - 10.1016/0378-1119(95)00507-3
DO - 10.1016/0378-1119(95)00507-3
M3 - Article
C2 - 8522173
AN - SCOPUS:0028839703
VL - 165
SP - 183
EP - 189
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -