A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

Won Yong Song, Tomohiro Yamaki, Naoki Yamaji, Donghwi Ko, Ki Hong Jung, Miho Fujii-Kashino, Gynheung An, Enrico Martinoia, Youngsook Lee, Jian Feng

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.

Original languageEnglish
Pages (from-to)15699-15704
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number44
DOIs
Publication statusPublished - Nov 4 2014

Keywords

  • Abc transporter
  • Arsenic
  • Node
  • Rice
  • Vacuolar sequestration

ASJC Scopus subject areas

  • General

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