A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

Won Yong Song, Tomohiro Yamaki, Naoki Yamaji, Donghwi Ko, Ki Hong Jung, Miho Fujii-Kashino, Gynheung An, Enrico Martinoia, Youngsook Lee, Jian F eng Ma

Research output: Contribution to journalArticle

Abstract

Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.

Original languageEnglish
Pages (from-to)15699-15704
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number44
DOIs
Publication statusPublished - Nov 4 2014

Fingerprint

ATP-Binding Cassette Transporters
Arsenic
Phloem
Oryza
Phytochelatins
Food Chain
Poisons
Skin Neoplasms
Vacuoles
Cadmium
Blood Vessels
Yeasts

Keywords

  • ABC transporter
  • arsenic
  • node
  • rice
  • vacuolar sequestration

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain. / Song, Won Yong; Yamaki, Tomohiro; Yamaji, Naoki; Ko, Donghwi; Jung, Ki Hong; Fujii-Kashino, Miho; An, Gynheung; Martinoia, Enrico; Lee, Youngsook; Ma, Jian F eng.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 44, 04.11.2014, p. 15699-15704.

Research output: Contribution to journalArticle

Song, Won Yong ; Yamaki, Tomohiro ; Yamaji, Naoki ; Ko, Donghwi ; Jung, Ki Hong ; Fujii-Kashino, Miho ; An, Gynheung ; Martinoia, Enrico ; Lee, Youngsook ; Ma, Jian F eng. / A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 44. pp. 15699-15704.
@article{62b7d00e7e8a41ecb7606b4e0ce52373,
title = "A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain",
abstract = "Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.",
keywords = "ABC transporter, arsenic, node, rice, vacuolar sequestration",
author = "Song, {Won Yong} and Tomohiro Yamaki and Naoki Yamaji and Donghwi Ko and Jung, {Ki Hong} and Miho Fujii-Kashino and Gynheung An and Enrico Martinoia and Youngsook Lee and Ma, {Jian F eng}",
year = "2014",
month = "11",
day = "4",
doi = "10.1073/pnas.1414968111",
language = "English",
volume = "111",
pages = "15699--15704",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "44",

}

TY - JOUR

T1 - A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain

AU - Song, Won Yong

AU - Yamaki, Tomohiro

AU - Yamaji, Naoki

AU - Ko, Donghwi

AU - Jung, Ki Hong

AU - Fujii-Kashino, Miho

AU - An, Gynheung

AU - Martinoia, Enrico

AU - Lee, Youngsook

AU - Ma, Jian F eng

PY - 2014/11/4

Y1 - 2014/11/4

N2 - Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.

AB - Arsenic (As) is a chronic poison that causes severe skin lesions and cancer. Rice (Oryza sativa L.) is a major dietary source of As; therefore, reducing As accumulation in the rice grain and thereby diminishing the amount of As that enters the food chain is of critical importance. Here, we report that a member of the Oryza sativa C-type ATP-binding cassette (ABC) transporter (OsABCC) family, OsABCC1, is involved in the detoxification and reduction of As in rice grains. We found that OsABCC1 was expressed in many organs, including the roots, leaves, nodes, peduncle, and rachis. Expression was not affected when plants were exposed to low levels of As but was up-regulated in response to high levels of As. In both the basal nodes and upper nodes, which are connected to the panicle, OsABCC1 was localized to the phloem region of vascular bundles. Furthermore, OsABCC1 was localized to the tonoplast and conferred phytochelatin-dependent As resistance in yeast. Knockout of OsABCC1 in rice resulted in decreased tolerance to As, but did not affect cadmium toxicity. At the reproductive growth stage, the As content was higher in the nodes and in other tissues of wild-type rice than in those of OsABCC1 knockout mutants, but was significantly lower in the grain. Taken together, our results indicate that OsABCC1 limits As transport to the grains by sequestering As in the vacuoles of the phloem companion cells of the nodes in rice.

KW - ABC transporter

KW - arsenic

KW - node

KW - rice

KW - vacuolar sequestration

UR - http://www.scopus.com/inward/record.url?scp=84927565253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927565253&partnerID=8YFLogxK

U2 - 10.1073/pnas.1414968111

DO - 10.1073/pnas.1414968111

M3 - Article

C2 - 25331872

VL - 111

SP - 15699

EP - 15704

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 44

ER -