A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis

Toshiyoshi Fujiwara, Elizabeth A. Grimm, Tapas Mukhopadhyay, De Wei Cai, Laurie B. Owen-Schaub, Jack A. Roth

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Multicellular tumor spheroids approximate the three-dimensional configuration of primary and metastatic tumors. The effects of retrovirusnediated transduction of wild-type p53 (wt-p53) were studied on multicellular tumor spheroids of human non-small cell lung cancer cell lines H322a, the p53 gene of which is homozygously mutated at codon 248, and WT226b, which has endogenous wt-p53. The growth of WT226b spheroids was not affected by exogenous wt-p53 transduction; the growth of H322a spheroids, however, was significantly inhibited by the addition of wt-p53 virus stocks. Transduction of cells by the wt-p53 retroviral vector and penetration of multiple cell layers in H322a spheroids was demonstrated by in situ polymerase chain reaction/hybridization with the neomycinresistant neo probe. Apoptotic changes indicating programmed cell death were observed in H322a spheroids treated with the wt-p53 virus. These results suggest that retroviral vectors can penetrate into multiple cell layers of three-dimensional tumor masses and induce potentially therapeutic effects.

Original languageEnglish
Pages (from-to)4129-4133
Number of pages5
JournalCancer Research
Volume53
Issue number18
Publication statusPublished - Sep 15 1993
Externally publishedYes

Fingerprint

Lung Neoplasms
Cellular Spheroids
Apoptosis
Growth
Neoplasms
Viruses
p53 Genes
Therapeutic Uses
Non-Small Cell Lung Carcinoma
Codon
Cell Death
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fujiwara, T., Grimm, E. A., Mukhopadhyay, T., Cai, D. W., Owen-Schaub, L. B., & Roth, J. A. (1993). A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis. Cancer Research, 53(18), 4129-4133.

A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis. / Fujiwara, Toshiyoshi; Grimm, Elizabeth A.; Mukhopadhyay, Tapas; Cai, De Wei; Owen-Schaub, Laurie B.; Roth, Jack A.

In: Cancer Research, Vol. 53, No. 18, 15.09.1993, p. 4129-4133.

Research output: Contribution to journalArticle

Fujiwara, T, Grimm, EA, Mukhopadhyay, T, Cai, DW, Owen-Schaub, LB & Roth, JA 1993, 'A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis', Cancer Research, vol. 53, no. 18, pp. 4129-4133.
Fujiwara, Toshiyoshi ; Grimm, Elizabeth A. ; Mukhopadhyay, Tapas ; Cai, De Wei ; Owen-Schaub, Laurie B. ; Roth, Jack A. / A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis. In: Cancer Research. 1993 ; Vol. 53, No. 18. pp. 4129-4133.
@article{f91d621b27f8408484bd556bc4ba2ba8,
title = "A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis",
abstract = "Multicellular tumor spheroids approximate the three-dimensional configuration of primary and metastatic tumors. The effects of retrovirusnediated transduction of wild-type p53 (wt-p53) were studied on multicellular tumor spheroids of human non-small cell lung cancer cell lines H322a, the p53 gene of which is homozygously mutated at codon 248, and WT226b, which has endogenous wt-p53. The growth of WT226b spheroids was not affected by exogenous wt-p53 transduction; the growth of H322a spheroids, however, was significantly inhibited by the addition of wt-p53 virus stocks. Transduction of cells by the wt-p53 retroviral vector and penetration of multiple cell layers in H322a spheroids was demonstrated by in situ polymerase chain reaction/hybridization with the neomycinresistant neo probe. Apoptotic changes indicating programmed cell death were observed in H322a spheroids treated with the wt-p53 virus. These results suggest that retroviral vectors can penetrate into multiple cell layers of three-dimensional tumor masses and induce potentially therapeutic effects.",
author = "Toshiyoshi Fujiwara and Grimm, {Elizabeth A.} and Tapas Mukhopadhyay and Cai, {De Wei} and Owen-Schaub, {Laurie B.} and Roth, {Jack A.}",
year = "1993",
month = "9",
day = "15",
language = "English",
volume = "53",
pages = "4129--4133",
journal = "Journal of Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "18",

}

TY - JOUR

T1 - A retroviral wild-type p53 expression vector penetrates human lung cancer spheroids and inhibits growth by inducing apoptosis

AU - Fujiwara, Toshiyoshi

AU - Grimm, Elizabeth A.

AU - Mukhopadhyay, Tapas

AU - Cai, De Wei

AU - Owen-Schaub, Laurie B.

AU - Roth, Jack A.

PY - 1993/9/15

Y1 - 1993/9/15

N2 - Multicellular tumor spheroids approximate the three-dimensional configuration of primary and metastatic tumors. The effects of retrovirusnediated transduction of wild-type p53 (wt-p53) were studied on multicellular tumor spheroids of human non-small cell lung cancer cell lines H322a, the p53 gene of which is homozygously mutated at codon 248, and WT226b, which has endogenous wt-p53. The growth of WT226b spheroids was not affected by exogenous wt-p53 transduction; the growth of H322a spheroids, however, was significantly inhibited by the addition of wt-p53 virus stocks. Transduction of cells by the wt-p53 retroviral vector and penetration of multiple cell layers in H322a spheroids was demonstrated by in situ polymerase chain reaction/hybridization with the neomycinresistant neo probe. Apoptotic changes indicating programmed cell death were observed in H322a spheroids treated with the wt-p53 virus. These results suggest that retroviral vectors can penetrate into multiple cell layers of three-dimensional tumor masses and induce potentially therapeutic effects.

AB - Multicellular tumor spheroids approximate the three-dimensional configuration of primary and metastatic tumors. The effects of retrovirusnediated transduction of wild-type p53 (wt-p53) were studied on multicellular tumor spheroids of human non-small cell lung cancer cell lines H322a, the p53 gene of which is homozygously mutated at codon 248, and WT226b, which has endogenous wt-p53. The growth of WT226b spheroids was not affected by exogenous wt-p53 transduction; the growth of H322a spheroids, however, was significantly inhibited by the addition of wt-p53 virus stocks. Transduction of cells by the wt-p53 retroviral vector and penetration of multiple cell layers in H322a spheroids was demonstrated by in situ polymerase chain reaction/hybridization with the neomycinresistant neo probe. Apoptotic changes indicating programmed cell death were observed in H322a spheroids treated with the wt-p53 virus. These results suggest that retroviral vectors can penetrate into multiple cell layers of three-dimensional tumor masses and induce potentially therapeutic effects.

UR - http://www.scopus.com/inward/record.url?scp=0027818396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027818396&partnerID=8YFLogxK

M3 - Article

C2 - 8395976

AN - SCOPUS:0027818396

VL - 53

SP - 4129

EP - 4133

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0008-5472

IS - 18

ER -