A restriction fragment length polymorphism (RFLP) locus of rat (Rattus norvegicus) linked to the Cs-1 locus of rat linkage group XIII

Masayoshi Takahashi, Hiroshi Yokota, Dai Ayusawa, Michio Oishi, Tetsuo Kunieda

Research output: Contribution to journalArticle

Abstract

A novel restriction fragment length polymorphism (RFLP) in inbred rats was revealed by Southern blot analysis with a clone arbitrarily chosen from a rat genomic library as a probe. A clone named α403 showed interstrain variations in the length of the EcoRI and HindIII fragments. The EcoRI fragments were either 0.7 or 3 kb, those of HindIII were either 4.5 or 5 kb, and three types were identified as combinations of those fragments in 20 inbred rat strains. These types segregated in backcross progeny as codominant alleles. The locus for the RFLP was thus named A403. Analysis of linkage between the RFLP locus and 13 other loci reveal that the A403 locus was closely linked to the Cs-1 locus (15 ± 5.2%), which belongs to rat linkage group XIII.

Original languageEnglish
Pages (from-to)537-544
Number of pages8
JournalBiochemical Genetics
Volume30
Issue number9-10
DOIs
Publication statusPublished - Oct 1 1992
Externally publishedYes

Keywords

  • catalase locus
  • rat
  • restriction fragment length polymorphisms

ASJC Scopus subject areas

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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