A restriction fragment length polymorphism of a p53-related sequence in rat (Rattus norvegicus).

Tetsuo Kunieda, H. Ikadai, T. Imamichi, Y. Toyoda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Polymorphisms of the p53 tumor-suppressor gene and its related sequences were investigated among inbred rat strains. In a series of Southern blot hybridizations using the human p53 cDNA probe with various restriction endonucleases, HindIII digests and PstI digests showed interstrain variation in the length of hybridizing fragments. Distribution of the HindIII variant fragments among 17 inbred rat strains was identical with that of PstI variant fragments. The variant fragments were segregated as codominant alleles in backcross progeny, and the locus for this restriction fragment length polymorphism of the p53-related sequence was found to correspond to a p53-related sequence located on rat chromosome 9.

Original languageEnglish
Pages (from-to)191-193
Number of pages3
JournalJournal of Veterinary Medical Science
Volume56
Issue number1
Publication statusPublished - Feb 1994
Externally publishedYes

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Inbred Strains Rats
Rattus norvegicus
Restriction Fragment Length Polymorphisms
Deoxyribonuclease HindIII
restriction fragment length polymorphism
Chromosomes, Human, Pair 9
DNA Restriction Enzymes
rats
Southern Blotting
Tumor Suppressor Genes
tumor suppressor genes
Complementary DNA
Alleles
restriction endonucleases
Southern blotting
hybridization
genetic polymorphism
alleles
chromosomes
loci

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

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A restriction fragment length polymorphism of a p53-related sequence in rat (Rattus norvegicus). / Kunieda, Tetsuo; Ikadai, H.; Imamichi, T.; Toyoda, Y.

In: Journal of Veterinary Medical Science, Vol. 56, No. 1, 02.1994, p. 191-193.

Research output: Contribution to journalArticle

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