A replicase of Potato virus X acts as the resistance-breaking determinant for JAX1-mediated resistance

Kyoko Sugawara, Takuya Shiraishi, Tetsuya Yoshida, Naoko Fujita, Osamu Netsu, Yasuyuki Yamaji, Shigetou Namba

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Lectin-mediated resistance (LMR) has been suggested to comprise an uncharacterized branch of antiviral plant innate immunity. To unveil the feature of resistance conferred by jacalin-type lectin required for potexvirus resistance 1 (JAX1), a recently isolated LMR gene against potexviruses, we analyzed the resistance-breaking variants to find the viral component involved in resistance. We employed grafting-mediated inoculation, a high-pressure virus inoculation method, to obtain Potato virus X (PVX) variants that can overcome JAX1-mediated resistance. Whole-genome sequencing of the variants suggested that a single amino acid in the methyl transferase domain of the replicase encoded by PVX is responsible for this resistance-breaking property. Reintroduction of the amino-acid substitution to avirulent wild-type PVX was sufficient to overcome the JAX1-mediated resistance. These results suggest that viral replicase is involved in JAX1-mediated resistance. The residue that determines the resistance-breaking properties was highly conserved among potexviruses, suggesting a general role of the residue in potexvirus-JAX1 interactions.

Original languageEnglish
Pages (from-to)1106-1112
Number of pages7
JournalMolecular Plant-Microbe Interactions
Volume26
Issue number9
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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