A Reovirus of the Fungus Cryphonectria parasitica That Is Infectious as Particles and Related to the Coltivirus Genus of Animal Pathogens

Bradley I. Hillman, S. Supyani, Hideki Kondo, Nobuhiro Suzuki

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

RNA viruses of filamentous fungi fall into two broad categories, those that contain double-stranded RNA (dsRNA) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded RNA viruses with dsRNA replicative intermediates found within lipid vesicles. Effective infectivity systems have been described for the latter, using RNA transcripts, but not for the former. We report the characterization of a reovirus from Cryphonectria parasitica, the filamentous fungus that causes chestnut blight disease. The virus substantially reduces the virulence of the fungus and results in dramatically altered colony morphology, as well as changes in other associated fungal traits, relative to the virus-free isogenic strain. Virus particles from infected mycelium contained 11 segments of dsRNA and showed characteristics typical of the family Reoviridae. Sequences of the largest three segments revealed that the virus is closely related to the Coltivirus genus of animal pathogens, which includes the human pathogen Colorado tickfever virus. The introduction of purified virus particles into protoplasts from virus-free isolates of the fungus resulted in a newly infected mycelium with the same morphology and virus composition as the original virus-infected isolate. This represents the completion of Koch's postulates for a true dsRNA virus from a filamentous fungus and the description of a definitive fungal member of the family Reoviridae.

Original languageEnglish
Pages (from-to)892-898
Number of pages7
JournalJournal of Virology
Volume78
Issue number2
DOIs
Publication statusPublished - Jan 2004

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Coltivirus
Cryphonectria parasitica
animal pathogens
Reoviridae
Fungi
Viruses
Double-Stranded RNA
viruses
fungi
RNA Viruses
double-stranded RNA
Mycelium
virion
Virion
mycelium
positive sense, single-stranded RNA viruses
dsRNA viruses
Protoplasts
messenger RNA
protoplasts

ASJC Scopus subject areas

  • Immunology

Cite this

A Reovirus of the Fungus Cryphonectria parasitica That Is Infectious as Particles and Related to the Coltivirus Genus of Animal Pathogens. / Hillman, Bradley I.; Supyani, S.; Kondo, Hideki; Suzuki, Nobuhiro.

In: Journal of Virology, Vol. 78, No. 2, 01.2004, p. 892-898.

Research output: Contribution to journalArticle

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