A rapid microfluidic switching system for analysis at the single cellular level

Akira Yamada, Yuki Katanosaka, Satoshi Mohri, Keiji Naruse

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Analysis of cellular responses to chemicals at high spatiotemporal resolution is required for precise understanding of intracellular signal transduction. Here, we demonstrated a novel method for applying different solutions to a part of or all of a cell at high spatiotemporal resolution. We fabricated a microfluidic device using polydimethylsiloxane, and the sharp interface between the two solution streams flowing in the channel was used for the application of different solutions. We constructed a computer-controlled system to control the interface movement precisely, rapidly, and reproducibly during positioning, and spatial and temporal resolutions attained were 1.6 μm and 189 ms, respectively. We then applied the present system to the analysis of intracellular responses to chemicals. We were able to measure [Ca 2+] increases within 500 ms, when one laminar stream covered a part of the cell. This method can be used as a generic platform to investigate responses against drugs at the single cell level.

Original languageEnglish
Article number5401110
Pages (from-to)306-311
Number of pages6
JournalIEEE Transactions on Nanobioscience
Volume8
Issue number4
DOIs
Publication statusPublished - Dec 2009

Keywords

  • Adenosine 5'-triphosphate (ATP)
  • Adherent
  • Human embryonic kidney 293 (HEK 293)
  • Laminar flow
  • Microfluidic device
  • Perfusion
  • Polydimethylsiloxane (PDMS)
  • Single cell

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

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