TY - JOUR
T1 - A rapid microfluidic switching system for analysis at the single cellular level
AU - Yamada, Akira
AU - Katanosaka, Yuki
AU - Mohri, Satoshi
AU - Naruse, Keiji
N1 - Funding Information:
Manuscript received April 9, 2009; revised August 8, 2009. Current version published February 3, 2010. This work was supported in part by a grant-in-aid for Scientific Research on Priority Areas “System Cell Engineering by Multi-Scale Manipulation,” Japan under Grant 17076006. Asterisk indicates corresponding author.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2009/12
Y1 - 2009/12
N2 - Analysis of cellular responses to chemicals at high spatiotemporal resolution is required for precise understanding of intracellular signal transduction. Here, we demonstrated a novel method for applying different solutions to a part of or all of a cell at high spatiotemporal resolution. We fabricated a microfluidic device using polydimethylsiloxane, and the sharp interface between the two solution streams flowing in the channel was used for the application of different solutions. We constructed a computer-controlled system to control the interface movement precisely, rapidly, and reproducibly during positioning, and spatial and temporal resolutions attained were 1.6 μm and 189 ms, respectively. We then applied the present system to the analysis of intracellular responses to chemicals. We were able to measure [Ca 2+] increases within 500 ms, when one laminar stream covered a part of the cell. This method can be used as a generic platform to investigate responses against drugs at the single cell level.
AB - Analysis of cellular responses to chemicals at high spatiotemporal resolution is required for precise understanding of intracellular signal transduction. Here, we demonstrated a novel method for applying different solutions to a part of or all of a cell at high spatiotemporal resolution. We fabricated a microfluidic device using polydimethylsiloxane, and the sharp interface between the two solution streams flowing in the channel was used for the application of different solutions. We constructed a computer-controlled system to control the interface movement precisely, rapidly, and reproducibly during positioning, and spatial and temporal resolutions attained were 1.6 μm and 189 ms, respectively. We then applied the present system to the analysis of intracellular responses to chemicals. We were able to measure [Ca 2+] increases within 500 ms, when one laminar stream covered a part of the cell. This method can be used as a generic platform to investigate responses against drugs at the single cell level.
KW - Adenosine 5'-triphosphate (ATP)
KW - Adherent
KW - Human embryonic kidney 293 (HEK 293)
KW - Laminar flow
KW - Microfluidic device
KW - Perfusion
KW - Polydimethylsiloxane (PDMS)
KW - Single cell
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U2 - 10.1109/TNB.2009.2035253
DO - 10.1109/TNB.2009.2035253
M3 - Article
C2 - 20142146
AN - SCOPUS:76949101081
VL - 8
SP - 306
EP - 311
JO - IEEE Transactions on Nanobioscience
JF - IEEE Transactions on Nanobioscience
SN - 1536-1241
IS - 4
M1 - 5401110
ER -