A rapid in vitro assay system using anti-bromodeoxyuridine for drug susceptibility of plasmodium falciparum

Hiroyuki Doi, Akira Ishii, Kunio Shimono

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Drug effects were monitored by the measurement of bromodeoxyuridine (BrdU) incorporation into parasite deoxyribonucleic acid (DNA). Cells are pulse-labelled with BrdU and those which are synthesizing DNA incorporate BrdU into their DNA. Anti-BrdU is used to identify cells undergoing DNA synthesis at the time of the pulse. Concentration-effect curves were determined for chloroquine, pyrimethamine and quinine in culture for only 3 h in RPMI1640 medium supplemented with BrdU. Different strains of Plasmodium falciparum could be distinguished when BrdU uptake was monitored by the ELISA. This assay offers a fast and accurate method of monitoring the effects of a variety of antimalarial drugs.

Original languageEnglish
Pages (from-to)190-193
Number of pages4
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume82
Issue number2
DOIs
Publication statusPublished - Mar 1988

ASJC Scopus subject areas

  • Parasitology
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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