A rapid hydroponic screening for aluminium tolerance in barley

Jian Feng Ma, Shao Jian Zheng, Xiao Feng Li, Kazuyoshi Takeda, Hideaki Matsumoto

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Selection and breeding of crops for aluminium (Al) tolerance is a useful approach to increase production on acid soils. This requires a rapid and reliable system to discriminate between Al-tolerant and Al-sensitive genotypes. A hydroponic system was developed to screen for Al tolerance in barley (Hordeum vulgate L.) to overcome several problems encountered in previous screening methods. Four levels of Al (5, 10, 20, and 40 μM) in 1 mM CaCl2 solution at pH 4.5 were used to rank lines for Al-tolerance. Each line was cultured in a different compartment to eliminate chemical and pH interactions among lines. To avoid changes in Al tolerance due to other factors such as the calcium (Ca) concentration of the solution, Al-tolerant (Atlas 66) and Al-sensitive (Scout 66) cultivars of wheat (Triticum aestivum L.) were used as reference cultivars. Five ranks of Al tolerance from highly tolerant to highly sensitive were established by comparison with each reference. Eriochrome cyanine R staining was used for the rapid evaluation of Al tolerance. This screening system allowed classification of about 50 barley lines into five different Al tolerance groups within one week. Using this system, screening of ca. 600 barley lines from various regions of the world was conducted. Most lines were sensitive to Al, but ninety lines showed intermediate Al-tolerance. Thirty nine lines were highly sensitive to Al in solution.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalPlant and Soil
Volume191
Issue number1
DOIs
Publication statusPublished - 1997

Fingerprint

hydroponics
barley
aluminum
tolerance
screening
cultivar
Hordeum
cultivars
acid soil
plant breeding
acid soils
atlas

Keywords

  • Aluminium
  • Barley
  • Eriochrome cyanine R staining
  • Hordeum vulgate L. screening

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Soil Science

Cite this

Ma, J. F., Zheng, S. J., Li, X. F., Takeda, K., & Matsumoto, H. (1997). A rapid hydroponic screening for aluminium tolerance in barley. Plant and Soil, 191(1), 133-137. https://doi.org/10.1023/A:1004257711952

A rapid hydroponic screening for aluminium tolerance in barley. / Ma, Jian Feng; Zheng, Shao Jian; Li, Xiao Feng; Takeda, Kazuyoshi; Matsumoto, Hideaki.

In: Plant and Soil, Vol. 191, No. 1, 1997, p. 133-137.

Research output: Contribution to journalArticle

Ma, JF, Zheng, SJ, Li, XF, Takeda, K & Matsumoto, H 1997, 'A rapid hydroponic screening for aluminium tolerance in barley', Plant and Soil, vol. 191, no. 1, pp. 133-137. https://doi.org/10.1023/A:1004257711952
Ma, Jian Feng ; Zheng, Shao Jian ; Li, Xiao Feng ; Takeda, Kazuyoshi ; Matsumoto, Hideaki. / A rapid hydroponic screening for aluminium tolerance in barley. In: Plant and Soil. 1997 ; Vol. 191, No. 1. pp. 133-137.
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