A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis

Kazutaka Makino, Takahisa Yano, Takayoshi Maiguma, Daisuke Teshima, Toshiaki Sendo, Yoshinori Itoh, Ryozo Oishi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A rapid and simultaneous determination of several analgesic antiinflammatory agents - ibuprofen, acetaminophen, indomethacin, and salicylic acid - in human serum was developed by using capillary zone electrophoresis (CZE) coupled with diode-array ultraviolet detection. After precipitation of serum protein with acetonitrile containing 3-isobutyl-1-methylxanthine as the internal standard, an aliquot of deproteinized samples was applied directly to the CZE system. It enabled us to measure all of these four agents within 6 min, and there were no peaks interfering with the assay of these agents or 3-isobutyl-1-methylxanthine. Both the separation and quantification of these agents in human serum were reproducible after repeated analysis within a day or day-to-day analysis. In addition, there was a good correlation for each drug (r = 0.997-0.999) between the values in serum determined by CZE analysis and those measured either by high-performance liquid chromatography with ultraviolet detection (ibuprofen and indomethacin) or by fluorescence polarization immunoassay (acetaminophen and salicylic acid). Therefore, the present CZE analysis could provide a simple, rapid, and efficient method for the identification as well as monitoring of analgesic antiinflammatory agents, particularly in serum of patients suffering from intoxication by overdosage of these agents.

Original languageEnglish
Pages (from-to)574-580
Number of pages7
JournalTherapeutic Drug Monitoring
Volume25
Issue number5
DOIs
Publication statusPublished - Oct 1 2003
Externally publishedYes

Keywords

  • Acetaminophen
  • Acetonitrile deproteinization
  • Capillary electrophoresis
  • Ibuprofen
  • Indomethacin
  • Salicylic acid
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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