A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis

Kazutaka Makino, Takahisa Yano, Takayoshi Maiguma, Daisuke Teshima, Toshiaki Sendo, Yoshinori Itoh, Ryozo Oishi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A rapid and simultaneous determination of several analgesic antiinflammatory agents - ibuprofen, acetaminophen, indomethacin, and salicylic acid - in human serum was developed by using capillary zone electrophoresis (CZE) coupled with diode-array ultraviolet detection. After precipitation of serum protein with acetonitrile containing 3-isobutyl-1-methylxanthine as the internal standard, an aliquot of deproteinized samples was applied directly to the CZE system. It enabled us to measure all of these four agents within 6 min, and there were no peaks interfering with the assay of these agents or 3-isobutyl-1-methylxanthine. Both the separation and quantification of these agents in human serum were reproducible after repeated analysis within a day or day-to-day analysis. In addition, there was a good correlation for each drug (r = 0.997-0.999) between the values in serum determined by CZE analysis and those measured either by high-performance liquid chromatography with ultraviolet detection (ibuprofen and indomethacin) or by fluorescence polarization immunoassay (acetaminophen and salicylic acid). Therefore, the present CZE analysis could provide a simple, rapid, and efficient method for the identification as well as monitoring of analgesic antiinflammatory agents, particularly in serum of patients suffering from intoxication by overdosage of these agents.

Original languageEnglish
Pages (from-to)574-580
Number of pages7
JournalTherapeutic Drug Monitoring
Volume25
Issue number5
DOIs
Publication statusPublished - Oct 2003
Externally publishedYes

Fingerprint

Capillary Electrophoresis
Electrophoresis
Analgesics
Anti-Inflammatory Agents
1-Methyl-3-isobutylxanthine
Salicylic Acid
Ibuprofen
Acetaminophen
Serum
Indomethacin
Fluorescence Polarization Immunoassay
High performance liquid chromatography
Blood Proteins
Assays
Diodes
Fluorescence
High Pressure Liquid Chromatography
Polarization
Monitoring
Pharmaceutical Preparations

Keywords

  • Acetaminophen
  • Acetonitrile deproteinization
  • Capillary electrophoresis
  • Ibuprofen
  • Indomethacin
  • Salicylic acid
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Pharmacology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology (medical)
  • Public Health, Environmental and Occupational Health

Cite this

A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis. / Makino, Kazutaka; Yano, Takahisa; Maiguma, Takayoshi; Teshima, Daisuke; Sendo, Toshiaki; Itoh, Yoshinori; Oishi, Ryozo.

In: Therapeutic Drug Monitoring, Vol. 25, No. 5, 10.2003, p. 574-580.

Research output: Contribution to journalArticle

Makino, Kazutaka ; Yano, Takahisa ; Maiguma, Takayoshi ; Teshima, Daisuke ; Sendo, Toshiaki ; Itoh, Yoshinori ; Oishi, Ryozo. / A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis. In: Therapeutic Drug Monitoring. 2003 ; Vol. 25, No. 5. pp. 574-580.
@article{49cfecc74eb147958bf2cd2f3d2fbb20,
title = "A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis",
abstract = "A rapid and simultaneous determination of several analgesic antiinflammatory agents - ibuprofen, acetaminophen, indomethacin, and salicylic acid - in human serum was developed by using capillary zone electrophoresis (CZE) coupled with diode-array ultraviolet detection. After precipitation of serum protein with acetonitrile containing 3-isobutyl-1-methylxanthine as the internal standard, an aliquot of deproteinized samples was applied directly to the CZE system. It enabled us to measure all of these four agents within 6 min, and there were no peaks interfering with the assay of these agents or 3-isobutyl-1-methylxanthine. Both the separation and quantification of these agents in human serum were reproducible after repeated analysis within a day or day-to-day analysis. In addition, there was a good correlation for each drug (r = 0.997-0.999) between the values in serum determined by CZE analysis and those measured either by high-performance liquid chromatography with ultraviolet detection (ibuprofen and indomethacin) or by fluorescence polarization immunoassay (acetaminophen and salicylic acid). Therefore, the present CZE analysis could provide a simple, rapid, and efficient method for the identification as well as monitoring of analgesic antiinflammatory agents, particularly in serum of patients suffering from intoxication by overdosage of these agents.",
keywords = "Acetaminophen, Acetonitrile deproteinization, Capillary electrophoresis, Ibuprofen, Indomethacin, Salicylic acid, Therapeutic drug monitoring",
author = "Kazutaka Makino and Takahisa Yano and Takayoshi Maiguma and Daisuke Teshima and Toshiaki Sendo and Yoshinori Itoh and Ryozo Oishi",
year = "2003",
month = "10",
doi = "10.1097/00007691-200310000-00006",
language = "English",
volume = "25",
pages = "574--580",
journal = "Therapeutic Drug Monitoring",
issn = "0163-4356",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis

AU - Makino, Kazutaka

AU - Yano, Takahisa

AU - Maiguma, Takayoshi

AU - Teshima, Daisuke

AU - Sendo, Toshiaki

AU - Itoh, Yoshinori

AU - Oishi, Ryozo

PY - 2003/10

Y1 - 2003/10

N2 - A rapid and simultaneous determination of several analgesic antiinflammatory agents - ibuprofen, acetaminophen, indomethacin, and salicylic acid - in human serum was developed by using capillary zone electrophoresis (CZE) coupled with diode-array ultraviolet detection. After precipitation of serum protein with acetonitrile containing 3-isobutyl-1-methylxanthine as the internal standard, an aliquot of deproteinized samples was applied directly to the CZE system. It enabled us to measure all of these four agents within 6 min, and there were no peaks interfering with the assay of these agents or 3-isobutyl-1-methylxanthine. Both the separation and quantification of these agents in human serum were reproducible after repeated analysis within a day or day-to-day analysis. In addition, there was a good correlation for each drug (r = 0.997-0.999) between the values in serum determined by CZE analysis and those measured either by high-performance liquid chromatography with ultraviolet detection (ibuprofen and indomethacin) or by fluorescence polarization immunoassay (acetaminophen and salicylic acid). Therefore, the present CZE analysis could provide a simple, rapid, and efficient method for the identification as well as monitoring of analgesic antiinflammatory agents, particularly in serum of patients suffering from intoxication by overdosage of these agents.

AB - A rapid and simultaneous determination of several analgesic antiinflammatory agents - ibuprofen, acetaminophen, indomethacin, and salicylic acid - in human serum was developed by using capillary zone electrophoresis (CZE) coupled with diode-array ultraviolet detection. After precipitation of serum protein with acetonitrile containing 3-isobutyl-1-methylxanthine as the internal standard, an aliquot of deproteinized samples was applied directly to the CZE system. It enabled us to measure all of these four agents within 6 min, and there were no peaks interfering with the assay of these agents or 3-isobutyl-1-methylxanthine. Both the separation and quantification of these agents in human serum were reproducible after repeated analysis within a day or day-to-day analysis. In addition, there was a good correlation for each drug (r = 0.997-0.999) between the values in serum determined by CZE analysis and those measured either by high-performance liquid chromatography with ultraviolet detection (ibuprofen and indomethacin) or by fluorescence polarization immunoassay (acetaminophen and salicylic acid). Therefore, the present CZE analysis could provide a simple, rapid, and efficient method for the identification as well as monitoring of analgesic antiinflammatory agents, particularly in serum of patients suffering from intoxication by overdosage of these agents.

KW - Acetaminophen

KW - Acetonitrile deproteinization

KW - Capillary electrophoresis

KW - Ibuprofen

KW - Indomethacin

KW - Salicylic acid

KW - Therapeutic drug monitoring

UR - http://www.scopus.com/inward/record.url?scp=0141923067&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141923067&partnerID=8YFLogxK

U2 - 10.1097/00007691-200310000-00006

DO - 10.1097/00007691-200310000-00006

M3 - Article

C2 - 14508380

AN - SCOPUS:0141923067

VL - 25

SP - 574

EP - 580

JO - Therapeutic Drug Monitoring

JF - Therapeutic Drug Monitoring

SN - 0163-4356

IS - 5

ER -