A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements

Toru Ikeda, Ryoko Shinohata, Masaaki Murakami, Kazuyoshi Hina, Shigeshi Kamikawa, Satoshi Hirohata, Shozo Kusachi, Arisa Tamura, Shinichi Usui

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background High-density lipoprotein (HDL) containing apolipoprotein E (apoE-rich HDL) represents only a small portion of plasma HDL. Reliable methods for determining and isolating apoE-rich HDL have not been well studied. Methods We established a novel analytical method for apoE-rich HDL using polyethylene glycol and a cation-exchange column (PEG-column method). Furthermore, we examined biochemical correlates of apoE-rich HDL-cholesterol (HDL-C) in 36 patients who underwent coronary computed tomographic angiography. Results Our PEG-column method demonstrated high reproducibility (coefficient of variation < 3.52%) and linearity up to 15 mg/dl for apoE-rich HDL-C concentrations. Isolated apoE-rich HDL exhibited a larger diameter (14.8 nm) than apoE-poor HDL (10.8 nm) and contained both apoE and apoA-I. ApoE-rich HDL-C concentrations correlated significantly with triglycerides (rs = − 0.646), LDL size (rs = 0.472), adiponectin (rs = 0.476), and other lipoprotein components. No significant correlation was obtained with the coronary calcium score. Multiple regression analysis revealed that plasma triglycerides and adiponectin concentrations remained significant independent predictors of apoE-rich (adjusted R2 = 0.486) but not apoE-poor HDL-C. Conclusions The PEG-column method demonstrated, to various degrees, significant correlations between HDL subfractions and several lipid-related biomarkers involved in an atherogenic lipoprotein profile. Our separation technique for apoE-rich HDL is useful to clarify the role of apoE-rich HDL in atherosclerosis.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalClinica Chimica Acta
Volume465
DOIs
Publication statusPublished - Feb 1 2017

Keywords

  • Atherogenic lipoprotein
  • Chromatography
  • HDL subfraction

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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