A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature

Hiroshi Yukawa; Masazumi Fujiwara; Kaori Kobayashi; Yuka Kumon; Kazu Miyaji; Yushi Nishimura; Keisuke Oshimi; Yumi Umehara; Yoshio Teki; Takayuki Iwasaki; Mutsuko Hatano; Hideki Hashimoto; Yoshinobu Baba

doi:10.1039/d0na00146e

A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature

Hiroshi Yukawa, Masazumi Fujiwara, Kaori Kobayashi, Yuka Kumon, Kazu Miyaji, Yushi Nishimura, Keisuke Oshimi, Yumi Umehara, Yoshio Teki, Takayuki Iwasaki, Mutsuko Hatano, Hideki Hashimoto, Yoshinobu Baba

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25 Citations (Scopus)

Abstract

Intracellular thermometry techniques play an important role in elucidating the relationship between the intracellular temperature and stem cell functions. However, there have been few reports on thermometry techniques that can detect the intracellular temperature of cells during several days of incubation. In this study, we developed a novel quantum thermometric sensing and analysis system (QTAS) using fluorescent nanodiamonds (FNDs). FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h of incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 μg mL⁻¹of FNDs. The peak of FNDs was confirmed at approximately 2.87 GHz with the characteristic fluorescence spectra of NV centers that could be optically detected (optically detected magnetic resonance [ODMR]). The ODMR peak clearly shifted to the high-frequency side as the temperature decreased and gave a mean temperature dependence of −(77.6 ± 11.0) kHz °C⁻¹, thus the intracellular temperature of living ASCs during several days of culturing could be precisely measured using the QTAS. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that the QTAS can be used to investigate the relationship between intracellular temperature and cellular functions.

Original language	English
Pages (from-to)	1859-1868
Number of pages	10
Journal	Nanoscale Advances
Volume	2
Issue number	5
DOIs	https://doi.org/10.1039/d0na00146e
Publication status	Published - May 2020
Externally published	Yes

ASJC Scopus subject areas

Engineering(all)
Bioengineering
Atomic and Molecular Physics, and Optics
Materials Science(all)
Chemistry(all)

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10.1039/d0na00146e

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Yukawa, H., Fujiwara, M., Kobayashi, K., Kumon, Y., Miyaji, K., Nishimura, Y., Oshimi, K., Umehara, Y., Teki, Y., Iwasaki, T., Hatano, M., Hashimoto, H., & Baba, Y. (2020). A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature. Nanoscale Advances, 2(5), 1859-1868. https://doi.org/10.1039/d0na00146e

Yukawa, H, Fujiwara, M, Kobayashi, K, Kumon, Y, Miyaji, K, Nishimura, Y, Oshimi, K, Umehara, Y, Teki, Y, Iwasaki, T, Hatano, M, Hashimoto, H & Baba, Y 2020, 'A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature', Nanoscale Advances, vol. 2, no. 5, pp. 1859-1868. https://doi.org/10.1039/d0na00146e

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title = "A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature",

abstract = "Intracellular thermometry techniques play an important role in elucidating the relationship between the intracellular temperature and stem cell functions. However, there have been few reports on thermometry techniques that can detect the intracellular temperature of cells during several days of incubation. In this study, we developed a novel quantum thermometric sensing and analysis system (QTAS) using fluorescent nanodiamonds (FNDs). FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h of incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 μg mL−1of FNDs. The peak of FNDs was confirmed at approximately 2.87 GHz with the characteristic fluorescence spectra of NV centers that could be optically detected (optically detected magnetic resonance [ODMR]). The ODMR peak clearly shifted to the high-frequency side as the temperature decreased and gave a mean temperature dependence of −(77.6 ± 11.0) kHz °C−1, thus the intracellular temperature of living ASCs during several days of culturing could be precisely measured using the QTAS. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that the QTAS can be used to investigate the relationship between intracellular temperature and cellular functions.",

author = "Hiroshi Yukawa and Masazumi Fujiwara and Kaori Kobayashi and Yuka Kumon and Kazu Miyaji and Yushi Nishimura and Keisuke Oshimi and Yumi Umehara and Yoshio Teki and Takayuki Iwasaki and Mutsuko Hatano and Hideki Hashimoto and Yoshinobu Baba",

note = "Funding Information: H. Y. acknowledges the nancial support from the JSPS-KAKENHI (No. 17H02731). H. Y. and M. F. thank the JSPS-KAKENHI for the joint project support (No. 16K13646). M. F. acknowledges the nancial support from the JSPS-KAKENHI (Nos 26706007, 26610077, and 17H02741), the MEXT-LEADER program, and Osaka City University (OCU-Strategic Research Grant 2017 & 2018 for young researchers and 2018 for top priority research). This work was also supported by a Grant-in-Aid for Scientic Research on Innovative Areas “Chemistry for Multimolecular Crowding Biosystems” (JSPS KAKENHI Grant No. 17H06354). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2020.",

year = "2020",

month = may,

doi = "10.1039/d0na00146e",

language = "English",

volume = "2",

pages = "1859--1868",

journal = "Nanoscale Advances",

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T1 - A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature

AU - Yukawa, Hiroshi

AU - Fujiwara, Masazumi

AU - Kobayashi, Kaori

AU - Kumon, Yuka

AU - Miyaji, Kazu

AU - Nishimura, Yushi

AU - Oshimi, Keisuke

AU - Umehara, Yumi

AU - Teki, Yoshio

AU - Iwasaki, Takayuki

AU - Hatano, Mutsuko

AU - Hashimoto, Hideki

AU - Baba, Yoshinobu

N1 - Funding Information: H. Y. acknowledges the nancial support from the JSPS-KAKENHI (No. 17H02731). H. Y. and M. F. thank the JSPS-KAKENHI for the joint project support (No. 16K13646). M. F. acknowledges the nancial support from the JSPS-KAKENHI (Nos 26706007, 26610077, and 17H02741), the MEXT-LEADER program, and Osaka City University (OCU-Strategic Research Grant 2017 & 2018 for young researchers and 2018 for top priority research). This work was also supported by a Grant-in-Aid for Scientic Research on Innovative Areas “Chemistry for Multimolecular Crowding Biosystems” (JSPS KAKENHI Grant No. 17H06354). Publisher Copyright: © The Royal Society of Chemistry 2020.

PY - 2020/5

Y1 - 2020/5

N2 - Intracellular thermometry techniques play an important role in elucidating the relationship between the intracellular temperature and stem cell functions. However, there have been few reports on thermometry techniques that can detect the intracellular temperature of cells during several days of incubation. In this study, we developed a novel quantum thermometric sensing and analysis system (QTAS) using fluorescent nanodiamonds (FNDs). FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h of incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 μg mL−1of FNDs. The peak of FNDs was confirmed at approximately 2.87 GHz with the characteristic fluorescence spectra of NV centers that could be optically detected (optically detected magnetic resonance [ODMR]). The ODMR peak clearly shifted to the high-frequency side as the temperature decreased and gave a mean temperature dependence of −(77.6 ± 11.0) kHz °C−1, thus the intracellular temperature of living ASCs during several days of culturing could be precisely measured using the QTAS. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that the QTAS can be used to investigate the relationship between intracellular temperature and cellular functions.

AB - Intracellular thermometry techniques play an important role in elucidating the relationship between the intracellular temperature and stem cell functions. However, there have been few reports on thermometry techniques that can detect the intracellular temperature of cells during several days of incubation. In this study, we developed a novel quantum thermometric sensing and analysis system (QTAS) using fluorescent nanodiamonds (FNDs). FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h of incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 μg mL−1of FNDs. The peak of FNDs was confirmed at approximately 2.87 GHz with the characteristic fluorescence spectra of NV centers that could be optically detected (optically detected magnetic resonance [ODMR]). The ODMR peak clearly shifted to the high-frequency side as the temperature decreased and gave a mean temperature dependence of −(77.6 ± 11.0) kHz °C−1, thus the intracellular temperature of living ASCs during several days of culturing could be precisely measured using the QTAS. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that the QTAS can be used to investigate the relationship between intracellular temperature and cellular functions.

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A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature

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