A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

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Abstract

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1+ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).

Original languageEnglish
Article number101786
JournalSTAR Protocols
Volume3
Issue number4
DOIs
Publication statusPublished - Dec 16 2022

Keywords

  • Cell culture
  • Cell differentiation
  • Developmental biology
  • Organoids
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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