A proteoglycan mediates inductive interaction during plant vascular development

Hiroyasu Motose, Munetaka Sugiyama, Hiroo Fukuda

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Inductive cell-cell interactions are essential for controlling cell fate determination in both plants and animals; however, the chemical basis of inductive signals in plants remains little understood. A proteoglycan-like factor named xylogen mediates local and inductive cell-cell interactions required for xylem differentiation in Zinnia cells cultured in vitro. Here we describe the purification of xylogen and cloning of its complementary DNA, and present evidence for its role in planta. The polypeptide backbone of xylogen is a hybrid-type molecule with properties of both arabinogalactan proteins and nonspecific lipid-transfer proteins. Xylogen predominantly accumulates in the meristem, procambium and xylem. In the xylem, xylogen has a polar localization in the cell walls of differentiating tracheary elements. Double knockouts of Arabidopsis lacking both genes that encode xylogen proteins show defects in vascular development: discontinuous veins, improperly interconnected vessel elements and simplified venation. Our results suggest that the polar secretion of xylogen draws neighbouring cells into the pathway of vascular differentiation to direct continuous vascular development, thereby identifying a molecule that mediates an inductive cell-cell interaction involved in plant tissue differentiation.

Original languageEnglish
Pages (from-to)873-878
Number of pages6
JournalNature
Volume429
Issue number6994
DOIs
Publication statusPublished - Jun 24 2004
Externally publishedYes

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Plant Development
Proteoglycans
Blood Vessels
Xylem
Cell Communication
Meristem
Arabidopsis
Cell Wall
Organism Cloning
Veins
Cultured Cells
Complementary DNA
Peptides
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

A proteoglycan mediates inductive interaction during plant vascular development. / Motose, Hiroyasu; Sugiyama, Munetaka; Fukuda, Hiroo.

In: Nature, Vol. 429, No. 6994, 24.06.2004, p. 873-878.

Research output: Contribution to journalArticle

Motose, Hiroyasu ; Sugiyama, Munetaka ; Fukuda, Hiroo. / A proteoglycan mediates inductive interaction during plant vascular development. In: Nature. 2004 ; Vol. 429, No. 6994. pp. 873-878.
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