A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

M. Noda, T. Asai, Toshinori Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original languageEnglish
Title of host publicationProcedia Chemistry
Pages1295-1298
Number of pages4
Volume1
Edition1
DOIs
Publication statusPublished - Sep 2009
Externally publishedYes
EventEurosensors 23rd Conference - Lausanne, Switzerland
Duration: Sep 6 2009Sep 9 2009

Other

OtherEurosensors 23rd Conference
CountrySwitzerland
CityLausanne
Period9/6/099/9/09

Fingerprint

Carbonic anhydrase
Microsensors
Carbonic Anhydrases
Liposomes
Leakage currents
Electrolytes
Biomolecules
Proteins
Micromachining

Keywords

  • Electrolyte
  • Interaction
  • Leakage current
  • Liposome
  • Protein

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Noda, M., Asai, T., Shimanouchi, T., Yamashita, K., Umakoshi, H., Okuyama, M., & Kuboi, R. (2009). A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein. In Procedia Chemistry (1 ed., Vol. 1, pp. 1295-1298) https://doi.org/10.1016/j.proche.2009.07.323

A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein. / Noda, M.; Asai, T.; Shimanouchi, Toshinori; Yamashita, K.; Umakoshi, H.; Okuyama, M.; Kuboi, R.

Procedia Chemistry. Vol. 1 1. ed. 2009. p. 1295-1298.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Noda, M, Asai, T, Shimanouchi, T, Yamashita, K, Umakoshi, H, Okuyama, M & Kuboi, R 2009, A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein. in Procedia Chemistry. 1 edn, vol. 1, pp. 1295-1298, Eurosensors 23rd Conference, Lausanne, Switzerland, 9/6/09. https://doi.org/10.1016/j.proche.2009.07.323
Noda, M. ; Asai, T. ; Shimanouchi, Toshinori ; Yamashita, K. ; Umakoshi, H. ; Okuyama, M. ; Kuboi, R. / A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein. Procedia Chemistry. Vol. 1 1. ed. 2009. pp. 1295-1298
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