Abstract
We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
| Original language | English |
|---|---|
| Title of host publication | Procedia Chemistry |
| Pages | 1295-1298 |
| Number of pages | 4 |
| Volume | 1 |
| Edition | 1 |
| DOIs | |
| Publication status | Published - Sep 2009 |
| Externally published | Yes |
| Event | Eurosensors 23rd Conference - Lausanne, Switzerland Duration: Sep 6 2009 → Sep 9 2009 |
Other
| Other | Eurosensors 23rd Conference |
|---|---|
| Country | Switzerland |
| City | Lausanne |
| Period | 9/6/09 → 9/9/09 |
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Keywords
- Electrolyte
- Interaction
- Leakage current
- Liposome
- Protein
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
Cite this
A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein. / Noda, M.; Asai, T.; Shimanouchi, Toshinori; Yamashita, K.; Umakoshi, H.; Okuyama, M.; Kuboi, R.
Procedia Chemistry. Vol. 1 1. ed. 2009. p. 1295-1298.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein
AU - Noda, M.
AU - Asai, T.
AU - Shimanouchi, Toshinori
AU - Yamashita, K.
AU - Umakoshi, H.
AU - Okuyama, M.
AU - Kuboi, R.
PY - 2009/9
Y1 - 2009/9
N2 - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
AB - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
KW - Electrolyte
KW - Interaction
KW - Leakage current
KW - Liposome
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=71549155285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71549155285&partnerID=8YFLogxK
U2 - 10.1016/j.proche.2009.07.323
DO - 10.1016/j.proche.2009.07.323
M3 - Conference contribution
AN - SCOPUS:71549155285
VL - 1
SP - 1295
EP - 1298
BT - Procedia Chemistry
ER -