A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

M. Noda; T. Asai; T. Shimanouchi; K. Yamashita; H. Umakoshi; M. Okuyama; R. Kuboi

doi:10.1016/j.proche.2009.07.323

A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K₄[Fe(CN)₆] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original language	English
Pages (from-to)	1295-1298
Number of pages	4
Journal	Procedia Chemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.323
Publication status	Published - Sep 2009
Externally published	Yes
Event	Eurosensors 23rd Conference - Lausanne, Switzerland Duration: Sep 6 2009 → Sep 9 2009

Keywords

Electrolyte
Interaction
Leakage current
Liposome
Protein

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.1016/j.proche.2009.07.323

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title = "A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein",

abstract = "We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.",

keywords = "Electrolyte, Interaction, Leakage current, Liposome, Protein",

author = "M. Noda and T. Asai and T. Shimanouchi and K. Yamashita and H. Umakoshi and M. Okuyama and R. Kuboi",

note = "Funding Information: This research was supported in part by Grants-in-Aid for Scientific Research (No. 19360162, 21246121, 20760539) from the Japan Society for the Promotion of Science. It was partly supported by the Core University Program between JSPS and VAST.; Eurosensors 23rd Conference ; Conference date: 06-09-2009 Through 09-09-2009",

year = "2009",

month = sep,

doi = "10.1016/j.proche.2009.07.323",

language = "English",

volume = "1",

pages = "1295--1298",

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AU - Noda, M.

AU - Asai, T.

AU - Shimanouchi, T.

AU - Yamashita, K.

AU - Umakoshi, H.

AU - Okuyama, M.

AU - Kuboi, R.

N1 - Funding Information: This research was supported in part by Grants-in-Aid for Scientific Research (No. 19360162, 21246121, 20760539) from the Japan Society for the Promotion of Science. It was partly supported by the Core University Program between JSPS and VAST.

PY - 2009/9

Y1 - 2009/9

N2 - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

AB - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

KW - Electrolyte

KW - Interaction

KW - Leakage current

KW - Liposome

KW - Protein

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DO - 10.1016/j.proche.2009.07.323

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AN - SCOPUS:71549155285

VL - 1

SP - 1295

EP - 1298

JO - Procedia Chemistry

JF - Procedia Chemistry

SN - 1876-6196

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T2 - Eurosensors 23rd Conference

Y2 - 6 September 2009 through 9 September 2009

ER -

A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

Abstract

Keywords

ASJC Scopus subject areas

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