A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

M. Noda; T. Asai; T. Shimanouchi; K. Yamashita; H. Umakoshi; M. Okuyama; R. Kuboi

doi:10.1016/j.proche.2009.07.323

A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

Research output: Contribution to journal › Conference article

4 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K₄[Fe(CN)₆] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original language	English
Pages (from-to)	1295-1298
Number of pages	4
Journal	Procedia Chemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.323
Publication status	Published - Sep 1 2009
Externally published	Yes
Event	Eurosensors 23rd Conference - Lausanne, Switzerland Duration: Sep 6 2009 → Sep 9 2009

Keywords

Electrolyte
Interaction
Leakage current
Liposome
Protein

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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Noda, M., Asai, T., Shimanouchi, T., Yamashita, K., Umakoshi, H., Okuyama, M., & Kuboi, R. (2009). A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein. Procedia Chemistry, 1(1), 1295-1298. https://doi.org/10.1016/j.proche.2009.07.323

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abstract = "We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.",

keywords = "Electrolyte, Interaction, Leakage current, Liposome, Protein",

author = "M. Noda and T. Asai and T. Shimanouchi and K. Yamashita and H. Umakoshi and M. Okuyama and R. Kuboi",

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AU - Noda, M.

AU - Asai, T.

AU - Shimanouchi, T.

AU - Yamashita, K.

AU - Umakoshi, H.

AU - Okuyama, M.

AU - Kuboi, R.

PY - 2009/9/1

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N2 - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

AB - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

KW - Electrolyte

KW - Interaction

KW - Leakage current

KW - Liposome

KW - Protein

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