A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

M. Noda, T. Asai, T. Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kuboi

Research output: Contribution to journalConference article

4 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original languageEnglish
Pages (from-to)1295-1298
Number of pages4
JournalProcedia Chemistry
Volume1
Issue number1
DOIs
Publication statusPublished - Sep 1 2009
Externally publishedYes
EventEurosensors 23rd Conference - Lausanne, Switzerland
Duration: Sep 6 2009Sep 9 2009

Keywords

  • Electrolyte
  • Interaction
  • Leakage current
  • Liposome
  • Protein

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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