A pressure-jump method as a new tool in growth physiology for monitoring physiological wall extensibility and effective turgor

Hisashi Okamoto, Qiang Liu, Kiyoshi Nakahori, Kiyoshi Katou

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A new method has been devised in order to allow the rapid determination of the physiological extensibility (Φ) of the cell wall together with the apparent effective turgor (P - Y'). The method consists of determining the elongation growth rate of a segment of pea hypocotyl with an auxanometer and applying a very small increase (around 10 kPa) in xylem pressure (jumps) by the xylem perfusion method. The cell turgor pressure is increased by the water surge from the xylem that is due to the pressure jump and reaches a new level within a short time. The elongation rate is also increased asymptotically to a new steady-state level within a short time. Φ is estimated by simply dividing the increase in the steady-state growth rate by the increase in perfusion pressure, which is monitored by a pressure transducer or by the increase in turgor pressure measured by a pressure probe. Minimal length of the time period required to get a new steady elongation rate is 1.5 min. Therefore, it is possible to scan continuously the change in Φ by the intermittent application of the brief, small pulses of xylem pressure during any experimental treatment. The apparent effective turgor P - Y' can be calculated by dividing the initial elongation growth rate by Φ Using this method, we have demonstrated experimentally the non-linearity of the relationship between elongation rate and turgor (Lockhart mechanical equation).

Original languageEnglish
Pages (from-to)979-985
Number of pages7
JournalPlant and Cell Physiology
Volume30
Issue number7
Publication statusPublished - Oct 1989
Externally publishedYes

Fingerprint

extensibility
Physiology
Physiologic Monitoring
turgor
physiology
Xylem
Jump
Monitoring
Pressure
Elongation
monitoring
xylem
Growth
Perfusion
methodology
Pressure Transducers
Hypocotyl
Peas
Pressure transducers
Cell Wall

Keywords

  • Pressure jump
  • Turgor
  • Vigna unguiculata
  • Wall extensibility
  • Xylem perfusion
  • Yield threshold

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

A pressure-jump method as a new tool in growth physiology for monitoring physiological wall extensibility and effective turgor. / Okamoto, Hisashi; Liu, Qiang; Nakahori, Kiyoshi; Katou, Kiyoshi.

In: Plant and Cell Physiology, Vol. 30, No. 7, 10.1989, p. 979-985.

Research output: Contribution to journalArticle

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