A Potent Insect Chitinase Inhibitor of Fungal Origin

Teruhiko Nitoda, Hirokazu Usuki, Hiroshi Kanzaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nM.

Original languageEnglish
Pages (from-to)891-894
Number of pages4
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume58
Issue number11-12
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Chitinase Inhibitor
  • Extracellular Polysaccharide
  • Sphaeropsis sp.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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