A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans

Keiko Kawauchi, Kiriko Shibutani, Hitoshi Yagisawa, Hideaki Kamata, Shin'ichi Nakatsuji, Hiroyuki Anzai, Yoshindo Yokoyama, Yoshinari Ikegami, Yoshinori Moriyama, Hajime Hirata

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.

Original languageEnglish
Pages (from-to)543-547
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume237
Issue number3
DOIs
Publication statusPublished - Aug 28 1997
Externally publishedYes

Fingerprint

Pseudoalteromonas
Immunosuppressive Agents
Prodigiosin
Thymidine
Adenosine Triphosphatases
Chromaffin Granules
Vacuolar Proton-Translocating ATPases
Membranes
Jurkat Cells
Proton-Translocating ATPases
T-cells
Ionophores
Cell proliferation
Therapeutic Uses
Concanavalin A
cycloprodigiosin
Pigments
Lipopolysaccharides
Phospholipids
Bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans. / Kawauchi, Keiko; Shibutani, Kiriko; Yagisawa, Hitoshi; Kamata, Hideaki; Nakatsuji, Shin'ichi; Anzai, Hiroyuki; Yokoyama, Yoshindo; Ikegami, Yoshinari; Moriyama, Yoshinori; Hirata, Hajime.

In: Biochemical and Biophysical Research Communications, Vol. 237, No. 3, 28.08.1997, p. 543-547.

Research output: Contribution to journalArticle

Kawauchi, K, Shibutani, K, Yagisawa, H, Kamata, H, Nakatsuji, S, Anzai, H, Yokoyama, Y, Ikegami, Y, Moriyama, Y & Hirata, H 1997, 'A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans', Biochemical and Biophysical Research Communications, vol. 237, no. 3, pp. 543-547. https://doi.org/10.1006/bbrc.1997.7186
Kawauchi, Keiko ; Shibutani, Kiriko ; Yagisawa, Hitoshi ; Kamata, Hideaki ; Nakatsuji, Shin'ichi ; Anzai, Hiroyuki ; Yokoyama, Yoshindo ; Ikegami, Yoshinari ; Moriyama, Yoshinori ; Hirata, Hajime. / A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 237, No. 3. pp. 543-547.
@article{c24d394a9bb84ad1b0ef8023965632bc,
title = "A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans",
abstract = "Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.",
author = "Keiko Kawauchi and Kiriko Shibutani and Hitoshi Yagisawa and Hideaki Kamata and Shin'ichi Nakatsuji and Hiroyuki Anzai and Yoshindo Yokoyama and Yoshinari Ikegami and Yoshinori Moriyama and Hajime Hirata",
year = "1997",
month = "8",
day = "28",
doi = "10.1006/bbrc.1997.7186",
language = "English",
volume = "237",
pages = "543--547",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans

AU - Kawauchi, Keiko

AU - Shibutani, Kiriko

AU - Yagisawa, Hitoshi

AU - Kamata, Hideaki

AU - Nakatsuji, Shin'ichi

AU - Anzai, Hiroyuki

AU - Yokoyama, Yoshindo

AU - Ikegami, Yoshinari

AU - Moriyama, Yoshinori

AU - Hirata, Hajime

PY - 1997/8/28

Y1 - 1997/8/28

N2 - Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.

AB - Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.

UR - http://www.scopus.com/inward/record.url?scp=0031589546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031589546&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.7186

DO - 10.1006/bbrc.1997.7186

M3 - Article

C2 - 9299400

AN - SCOPUS:0031589546

VL - 237

SP - 543

EP - 547

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -