Abstract
Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.
| Original language | English |
|---|---|
| Pages (from-to) | 543-547 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 237 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 28 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans. / Kawauchi, Keiko; Shibutani, Kiriko; Yagisawa, Hitoshi; Kamata, Hideaki; Nakatsuji, Shin'ichi; Anzai, Hiroyuki; Yokoyama, Yoshindo; Ikegami, Yoshinari; Moriyama, Yoshinori; Hirata, Hajime.
In: Biochemical and Biophysical Research Communications, Vol. 237, No. 3, 28.08.1997, p. 543-547.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans
AU - Kawauchi, Keiko
AU - Shibutani, Kiriko
AU - Yagisawa, Hitoshi
AU - Kamata, Hideaki
AU - Nakatsuji, Shin'ichi
AU - Anzai, Hiroyuki
AU - Yokoyama, Yoshindo
AU - Ikegami, Yoshinari
AU - Moriyama, Yoshinori
AU - Hirata, Hajime
PY - 1997/8/28
Y1 - 1997/8/28
N2 - Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.
AB - Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.
UR - http://www.scopus.com/inward/record.url?scp=0031589546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031589546&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.7186
DO - 10.1006/bbrc.1997.7186
M3 - Article
C2 - 9299400
AN - SCOPUS:0031589546
VL - 237
SP - 543
EP - 547
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -