A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans

Keiko Kawauchi, Kiriko Shibutani, Hitoshi Yagisawa, Hideaki Kamata, Shin'ichi Nakatsuji, Hiroyuki Anzai, Yoshindo Yokoyama, Yoshinari Ikegami, Yoshinori Moriyama, Hajime Hirata

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77 Citations (Scopus)

Abstract

Cycloprodigiosin hydrochloride (cPrG·HCl), a member of the prodigiosin family, is a red pigment obtained from the marine bacterium Pseudoalteromonas denitrificans. cPrG·HCl markedly suppressed 3H-thymidine incorporation by concanavalin A stimulated murine splenocytes but had little effect on lipopolysaccharide dependent 3H-thymidine incorporation, indicating that cPrG·HCl acts as a selective inhibitor of T cell proliferation in the same way as other members of the prodigiosin family. cPrG·HCl inhibited the proliferation of the PMA stimulated Jurkat cells through an apoptotic process. Intriguingly, cPrG·HCl inhibited the H+ translocation by vacuolar type ATPase in chromaffin granule membranes without any effect on either its ATPase activity nor on the membrane conductance of phospholipid bilayers, suggesting that cPrG·HCl selectively uncouples H+ translocation from the ATPase reaction rather than acting as a non-specific ionophore. Since crystalline cPrG·HCl is highly stable, it raises the possibility of its therapeutic use as an immunosuppressant.

Original languageEnglish
Pages (from-to)543-547
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume237
Issue number3
DOIs
Publication statusPublished - Aug 28 1997

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kawauchi, K., Shibutani, K., Yagisawa, H., Kamata, H., Nakatsuji, S., Anzai, H., Yokoyama, Y., Ikegami, Y., Moriyama, Y., & Hirata, H. (1997). A possible immunosuppressant, cycloprodigiosin hydrochloride, obtained from Pseudoalteromonas denitrificans. Biochemical and Biophysical Research Communications, 237(3), 543-547. https://doi.org/10.1006/bbrc.1997.7186