A polarly localized transporter for efficient manganese uptake in rice

Daisei Ueno, Akimasa Sasaki, Naoki Yamaji, Takaaki Miyaji, Yumi Fujii, Yuma Takemoto, Sawako Moriyama, Jing Che, Yoshinori Moriyama, Kozo Iwasaki, Jian Feng Ma

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.

Original languageEnglish
Article number15170
JournalNature Plants
Volume1
DOIs
Publication statusPublished - Nov 9 2015

Fingerprint

manganese
transporters
metal tolerance
uptake mechanisms
rice
endodermis
stele
proteins
shoots
cells
cations
Oryza sativa
plasma membrane
metals
Arabidopsis
plant growth
yeasts
soil

ASJC Scopus subject areas

  • Plant Science

Cite this

A polarly localized transporter for efficient manganese uptake in rice. / Ueno, Daisei; Sasaki, Akimasa; Yamaji, Naoki; Miyaji, Takaaki; Fujii, Yumi; Takemoto, Yuma; Moriyama, Sawako; Che, Jing; Moriyama, Yoshinori; Iwasaki, Kozo; Ma, Jian Feng.

In: Nature Plants, Vol. 1, 15170, 09.11.2015.

Research output: Contribution to journalArticle

Ueno, Daisei ; Sasaki, Akimasa ; Yamaji, Naoki ; Miyaji, Takaaki ; Fujii, Yumi ; Takemoto, Yuma ; Moriyama, Sawako ; Che, Jing ; Moriyama, Yoshinori ; Iwasaki, Kozo ; Ma, Jian Feng. / A polarly localized transporter for efficient manganese uptake in rice. In: Nature Plants. 2015 ; Vol. 1.
@article{f4b427a74dbf45b19ce58dd6e346a45d,
title = "A polarly localized transporter for efficient manganese uptake in rice",
abstract = "Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.",
author = "Daisei Ueno and Akimasa Sasaki and Naoki Yamaji and Takaaki Miyaji and Yumi Fujii and Yuma Takemoto and Sawako Moriyama and Jing Che and Yoshinori Moriyama and Kozo Iwasaki and Ma, {Jian Feng}",
year = "2015",
month = "11",
day = "9",
doi = "10.1038/nplants.2015.170",
language = "English",
volume = "1",
journal = "Nature Plants",
issn = "2055-026X",
publisher = "Palgrave Macmillan Ltd.",

}

TY - JOUR

T1 - A polarly localized transporter for efficient manganese uptake in rice

AU - Ueno, Daisei

AU - Sasaki, Akimasa

AU - Yamaji, Naoki

AU - Miyaji, Takaaki

AU - Fujii, Yumi

AU - Takemoto, Yuma

AU - Moriyama, Sawako

AU - Che, Jing

AU - Moriyama, Yoshinori

AU - Iwasaki, Kozo

AU - Ma, Jian Feng

PY - 2015/11/9

Y1 - 2015/11/9

N2 - Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.

AB - Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.

UR - http://www.scopus.com/inward/record.url?scp=84946866355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946866355&partnerID=8YFLogxK

U2 - 10.1038/nplants.2015.170

DO - 10.1038/nplants.2015.170

M3 - Article

VL - 1

JO - Nature Plants

JF - Nature Plants

SN - 2055-026X

M1 - 15170

ER -