Abstract
Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.
| Original language | English |
|---|---|
| Article number | 15170 |
| Journal | Nature Plants |
| Volume | 1 |
| DOIs | |
| Publication status | Published - Nov 9 2015 |
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ASJC Scopus subject areas
- Plant Science
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A polarly localized transporter for efficient manganese uptake in rice. / Ueno, Daisei; Sasaki, Akimasa; Yamaji, Naoki; Miyaji, Takaaki; Fujii, Yumi; Takemoto, Yuma; Moriyama, Sawako; Che, Jing; Moriyama, Yoshinori; Iwasaki, Kozo; Ma, Jian Feng.
In: Nature Plants, Vol. 1, 15170, 09.11.2015.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A polarly localized transporter for efficient manganese uptake in rice
AU - Ueno, Daisei
AU - Sasaki, Akimasa
AU - Yamaji, Naoki
AU - Miyaji, Takaaki
AU - Fujii, Yumi
AU - Takemoto, Yuma
AU - Moriyama, Sawako
AU - Che, Jing
AU - Moriyama, Yoshinori
AU - Iwasaki, Kozo
AU - Ma, Jian Feng
PY - 2015/11/9
Y1 - 2015/11/9
N2 - Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.
AB - Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.
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UR - http://www.scopus.com/inward/citedby.url?scp=84946866355&partnerID=8YFLogxK
U2 - 10.1038/nplants.2015.170
DO - 10.1038/nplants.2015.170
M3 - Article
VL - 1
JO - Nature Plants
JF - Nature Plants
SN - 2055-026X
M1 - 15170
ER -