A point mutation to the long terminal repeat of bovine leukemia virus related to viral productivity and transmissibility

Hironobu Murakami, Haruna Todaka, Jumpei Uchiyama, Reiichiro Sato, Kazuyuki Sogawa, Masahiro Sakaguchi, Kenji Tsukamoto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

It is important to establish the molecular basis of the high transmissibility of bovine leukemia virus (BLV) to develop new methods of preventing viral transmission. Hence, the aim of this study was to determine whether some strains had transmission advantages. First, we determined the whole BLV genome sequences of all 34 BLV-infected cows from one farm. Phylogenetic analysis divided strains into 26 major and 8 minor strains. The major strains dominantly spread independent of host factor, bovine leucocyte antigen. Further analysis, with molecular clones, associated transmissibility with viral productivity in vitro. In addition, the two groups could be classified by group-specific mutations. The reverse genetic approach demonstrated that a spontaneous mutation at nucleotide 175 of the BLV genome, which is located in the viral promoter region, could alter viral productivity by changing viral transactivation, suggesting that BLV transmissibility is affected by a spontaneous mutation associated with viral productivity.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalVirology
Volume537
DOIs
Publication statusPublished - Nov 2019
Externally publishedYes

Keywords

  • Bovine leukemia virus
  • Genetic variation
  • Molecular clone
  • Proviral load
  • Reverse genetics
  • Transmission

ASJC Scopus subject areas

  • Virology

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