A plasmidic class 1 integron from five Pseudomonas aeruginosa clinical strains harbored aacA4 and nonsense-mutated cmlA1 gene cassettes

He Yan, Lei Shi, Shinji Yamasaki, Xinhui Li, Yicheng Cao, Lin Li, Liansheng Yang, Shin-ichi Miyoshi

Research output: Contribution to journalArticle

Abstract

Five strains of multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa) were isolated from inpatients at a local hospital in China. The most frequent resistance was to cefoperazone, ciprofloxacin, ceftriaxone, cefotaxime, gentamicin, piperacillin, trimethoprim sulfamethoxazole, and tobramycin. These strains were found to contain the class 1 integron, in which the 2360-bp gene cassettes were flanked by 5′- and 3′-conserved segments. Sequence analysis revealed that the gene cassettes contained aacA4 and cmlA1 genes; however, the latter gene had a nonsense mutation resulting in the production of a truncated protein. To the best of our knowledge, this is the first report of a nonsense mutation in the cmlA1 gene. Moreover, the P. aeruginosa strains showed identical profiles in pulsed-field gel electrophoresis, suggesting that they were derived from the same clone. These results emphasize the importance of controlling the spread of multidrug-resistant pathogens in hospitals.

Original languageEnglish
Pages (from-to)750-755
Number of pages6
JournalJournal of Health Science
Volume53
Issue number6
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Integrons
Pseudomonas aeruginosa
Genes
Nonsense Codon
Cefoperazone
Piperacillin
Tobramycin
Cefotaxime
Ceftriaxone
Pulsed Field Gel Electrophoresis
Sulfamethoxazole Drug Combination Trimethoprim
Pathogens
Ciprofloxacin
Gentamicins
Electrophoresis
Sequence Analysis
Inpatients
China
Clone Cells
Gels

Keywords

  • Drug resistance
  • Integron
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

A plasmidic class 1 integron from five Pseudomonas aeruginosa clinical strains harbored aacA4 and nonsense-mutated cmlA1 gene cassettes. / Yan, He; Shi, Lei; Yamasaki, Shinji; Li, Xinhui; Cao, Yicheng; Li, Lin; Yang, Liansheng; Miyoshi, Shin-ichi.

In: Journal of Health Science, Vol. 53, No. 6, 12.2007, p. 750-755.

Research output: Contribution to journalArticle

Yan, He ; Shi, Lei ; Yamasaki, Shinji ; Li, Xinhui ; Cao, Yicheng ; Li, Lin ; Yang, Liansheng ; Miyoshi, Shin-ichi. / A plasmidic class 1 integron from five Pseudomonas aeruginosa clinical strains harbored aacA4 and nonsense-mutated cmlA1 gene cassettes. In: Journal of Health Science. 2007 ; Vol. 53, No. 6. pp. 750-755.
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AU - Miyoshi, Shin-ichi

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