A pistil-specific thaumatin/PR5-like protein gene of Japanese pear (Pyrus serotina): Sequence and promoter activity of the 5′ region in transgenic tobacco

Hidenori Sassa, Koichiro Ushijima, Hisashi Hirano

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The genomic clone encoding the pistil-specific thaumatin/PR5-like protein (PsTL1) was isolated from Japanese pear (Pyrus serotina). Sequence analysis showed that the genomic clone contained the 5′-flanking sequence of 2.4 kb, the 3′-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. A sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5′-flanking region of the PsTL1 gene. The 2.4 kb 5′-flanking region was fused to the GUS coding sequence and transferred to tobacco. Transgenic tobacco showed GUS activity in pistil and, at low level, in anther, but not in other floral organs and leaf. Histochemical analysis localized GUS activity to stigma, transmitting tissue, anther and pollen of transgenic tobacco.

Original languageEnglish
Pages (from-to)371-377
Number of pages7
JournalPlant Molecular Biology
Volume50
Issue number3
DOIs
Publication statusPublished - Oct 2002
Externally publishedYes

Fingerprint

Pyrus pyrifolia
Pyrus
Tobacco
5' Flanking Region
pistil
pears
tobacco
promoter regions
genetically modified organisms
anthers
Clone Cells
Genes
clones
3' Flanking Region
genomics
Proteins
genes
proteins
Conserved Sequence
Pollen

Keywords

  • Anther
  • Japanese pear
  • Promoter
  • Style
  • Thaumatin-like protein
  • Tobacco

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

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abstract = "The genomic clone encoding the pistil-specific thaumatin/PR5-like protein (PsTL1) was isolated from Japanese pear (Pyrus serotina). Sequence analysis showed that the genomic clone contained the 5′-flanking sequence of 2.4 kb, the 3′-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. A sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5′-flanking region of the PsTL1 gene. The 2.4 kb 5′-flanking region was fused to the GUS coding sequence and transferred to tobacco. Transgenic tobacco showed GUS activity in pistil and, at low level, in anther, but not in other floral organs and leaf. Histochemical analysis localized GUS activity to stigma, transmitting tissue, anther and pollen of transgenic tobacco.",
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T2 - Sequence and promoter activity of the 5′ region in transgenic tobacco

AU - Sassa, Hidenori

AU - Ushijima, Koichiro

AU - Hirano, Hisashi

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AB - The genomic clone encoding the pistil-specific thaumatin/PR5-like protein (PsTL1) was isolated from Japanese pear (Pyrus serotina). Sequence analysis showed that the genomic clone contained the 5′-flanking sequence of 2.4 kb, the 3′-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. A sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5′-flanking region of the PsTL1 gene. The 2.4 kb 5′-flanking region was fused to the GUS coding sequence and transferred to tobacco. Transgenic tobacco showed GUS activity in pistil and, at low level, in anther, but not in other floral organs and leaf. Histochemical analysis localized GUS activity to stigma, transmitting tissue, anther and pollen of transgenic tobacco.

KW - Anther

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KW - Promoter

KW - Style

KW - Thaumatin-like protein

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