Abstract
The genomic clone encoding the pistil-specific thaumatin/PR5-like protein (PsTL1) was isolated from Japanese pear (Pyrus serotina). Sequence analysis showed that the genomic clone contained the 5′-flanking sequence of 2.4 kb, the 3′-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. A sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5′-flanking region of the PsTL1 gene. The 2.4 kb 5′-flanking region was fused to the GUS coding sequence and transferred to tobacco. Transgenic tobacco showed GUS activity in pistil and, at low level, in anther, but not in other floral organs and leaf. Histochemical analysis localized GUS activity to stigma, transmitting tissue, anther and pollen of transgenic tobacco.
| Original language | English |
|---|---|
| Pages (from-to) | 371-377 |
| Number of pages | 7 |
| Journal | Plant Molecular Biology |
| Volume | 50 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 2002 |
| Externally published | Yes |
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Keywords
- Anther
- Japanese pear
- Promoter
- Style
- Thaumatin-like protein
- Tobacco
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
A pistil-specific thaumatin/PR5-like protein gene of Japanese pear (Pyrus serotina) : Sequence and promoter activity of the 5′ region in transgenic tobacco. / Sassa, Hidenori; Ushijima, Koichiro; Hirano, Hisashi.
In: Plant Molecular Biology, Vol. 50, No. 3, 10.2002, p. 371-377.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A pistil-specific thaumatin/PR5-like protein gene of Japanese pear (Pyrus serotina)
T2 - Sequence and promoter activity of the 5′ region in transgenic tobacco
AU - Sassa, Hidenori
AU - Ushijima, Koichiro
AU - Hirano, Hisashi
PY - 2002/10
Y1 - 2002/10
N2 - The genomic clone encoding the pistil-specific thaumatin/PR5-like protein (PsTL1) was isolated from Japanese pear (Pyrus serotina). Sequence analysis showed that the genomic clone contained the 5′-flanking sequence of 2.4 kb, the 3′-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. A sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5′-flanking region of the PsTL1 gene. The 2.4 kb 5′-flanking region was fused to the GUS coding sequence and transferred to tobacco. Transgenic tobacco showed GUS activity in pistil and, at low level, in anther, but not in other floral organs and leaf. Histochemical analysis localized GUS activity to stigma, transmitting tissue, anther and pollen of transgenic tobacco.
AB - The genomic clone encoding the pistil-specific thaumatin/PR5-like protein (PsTL1) was isolated from Japanese pear (Pyrus serotina). Sequence analysis showed that the genomic clone contained the 5′-flanking sequence of 2.4 kb, the 3′-flanking sequence of 648 bp and the coding region interrupted by a intron of 351 bp. A sequence motif conserved in some pistil self-incompatibility gene promoters of solanaceous and brassicaceous species was located in the 5′-flanking region of the PsTL1 gene. The 2.4 kb 5′-flanking region was fused to the GUS coding sequence and transferred to tobacco. Transgenic tobacco showed GUS activity in pistil and, at low level, in anther, but not in other floral organs and leaf. Histochemical analysis localized GUS activity to stigma, transmitting tissue, anther and pollen of transgenic tobacco.
KW - Anther
KW - Japanese pear
KW - Promoter
KW - Style
KW - Thaumatin-like protein
KW - Tobacco
UR - http://www.scopus.com/inward/record.url?scp=0036792343&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036792343&partnerID=8YFLogxK
U2 - 10.1023/A:1019866029629
DO - 10.1023/A:1019866029629
M3 - Article
C2 - 12369614
AN - SCOPUS:0036792343
VL - 50
SP - 371
EP - 377
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 3
ER -