A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms

Akinori Kiba, Kazuhiro Toyoda, Kazuaki Yoshioka, Kami Tsujimura, Hirotaka Takahashi, Yuki Ichinose, Tadahiro Takeda, Toshiaki Kato, Tomonori Shiraishi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Apyrases (E.C.3.6.1.5; NTP-NDPases) are distributed in the cytosol, nuclei, cytoskeleton, and on the surface of plant cells. Some may play an important role in signal transduction from exogenous stimuli. We previously found a protein of ca. 55-kDa (CWP-55) in an ATPase-rich fraction from the pea cell wall bound to the elicitor and supprescins (suppressors of defense) from pea pathogen Mycosphaerella pinodes. We cloned the cDNA of CWP-55 that coincided with PsAPY1, one of two NTPase clones in a pea cDNA library. An analysis with a green fluorescent protein fusion protein indicated that PsAPY1 was distributed in the cell wall, nucleus, and cytoplasm. The recombinant PsAPY1 expressed in Escherichia coli had ATP-hydrolyzing activity responsive not only to the elicitor and supprescins from the pea pathogen but also to other elicitors such as a bacterial harpin, a yeast extract, and a synthetic glycopeptide. Biotinylated fungal signal molecules were bound to the recombinant PsAPY1 specifically. Resonant mirror detection confirmed such binding characteristics of PsAPY1. Based on these results, we discuss the role of cell-wall-bound NTPases in recognizing and responding to microorganisms on the cell wall surface.

Original languageEnglish
Pages (from-to)238-246
Number of pages9
JournalJournal of General Plant Pathology
Volume72
Issue number4
DOIs
Publication statusPublished - Aug 2006

Fingerprint

peas
cell walls
microorganisms
Mycosphaerella pinodes
apyrase
glycopeptides
pathogens
yeast extract
cytoskeleton
green fluorescent protein
cytosol
cDNA libraries
adenosinetriphosphatase
signal transduction
cytoplasm
proteins
clones
Escherichia coli
elicitors
cells

Keywords

  • Binding protein
  • Cell wall
  • Elicitor
  • NTPase
  • Receptor
  • Suppressor of defense

ASJC Scopus subject areas

  • Plant Science

Cite this

A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms. / Kiba, Akinori; Toyoda, Kazuhiro; Yoshioka, Kazuaki; Tsujimura, Kami; Takahashi, Hirotaka; Ichinose, Yuki; Takeda, Tadahiro; Kato, Toshiaki; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 72, No. 4, 08.2006, p. 238-246.

Research output: Contribution to journalArticle

Kiba, A, Toyoda, K, Yoshioka, K, Tsujimura, K, Takahashi, H, Ichinose, Y, Takeda, T, Kato, T & Shiraishi, T 2006, 'A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms', Journal of General Plant Pathology, vol. 72, no. 4, pp. 238-246. https://doi.org/10.1007/s10327-006-0279-7
Kiba, Akinori ; Toyoda, Kazuhiro ; Yoshioka, Kazuaki ; Tsujimura, Kami ; Takahashi, Hirotaka ; Ichinose, Yuki ; Takeda, Tadahiro ; Kato, Toshiaki ; Shiraishi, Tomonori. / A pea NTPase, PsAPY1, recognizes signal molecules from microorganisms. In: Journal of General Plant Pathology. 2006 ; Vol. 72, No. 4. pp. 238-246.
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