A PDZ-Binding motif controls basolateral targeting of syndecan-1 along the biosynthetic pathway in polarized epithelial cells

Sandra Maday, Eric Anderson, Henry C. Chang, James Shorter, Ayano Satoh, Jeff Sfakianos, Heike Fölsch, James M. Anderson, Zenta Walther, Ira Mellman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The cell surface proteoglycan, syndecan-1, is essential for normal epithelial morphology and function. Syndecan-1 is selectively localized to the basolateral domain of polarized epithelial cells and interacts with cytosolic PDZ (PSD-95, discs large, ZO-1) domain-containing proteins. Here, we show that the polarity of syndecan-1 is determined by its type II PDZ-binding motif. Mutations within the PDZ-binding motif lead to the mislocalization of syndecan-1 to the apical surface. In contrast to previous examples, however, PDZ-binding motif-dependent polarity is not determined by retention at the basolateral surface but rather by polarized sorting prior to syndecan-1's arrival at the plasma membrane. Although none of the four known PDZ-binding partners of syndecan-1 appears to control basolateral localization, our results show that the PDZ-binding motif of syndecan-1 is decoded along the biosynthetic pathway establishing a potential role for PDZ-mediated interactions in polarized sorting.

Original languageEnglish
Pages (from-to)1915-1924
Number of pages10
JournalTraffic
Volume9
Issue number11
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Syndecan-1
Biosynthetic Pathways
Epithelial Cells
Sorting
Proteoglycans
Cell membranes
Cell Membrane
Mutation

Keywords

  • Basolateral
  • Epithelia
  • PDZ
  • Polarity
  • Syndecan-1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

A PDZ-Binding motif controls basolateral targeting of syndecan-1 along the biosynthetic pathway in polarized epithelial cells. / Maday, Sandra; Anderson, Eric; Chang, Henry C.; Shorter, James; Satoh, Ayano; Sfakianos, Jeff; Fölsch, Heike; Anderson, James M.; Walther, Zenta; Mellman, Ira.

In: Traffic, Vol. 9, No. 11, 2008, p. 1915-1924.

Research output: Contribution to journalArticle

Maday, S, Anderson, E, Chang, HC, Shorter, J, Satoh, A, Sfakianos, J, Fölsch, H, Anderson, JM, Walther, Z & Mellman, I 2008, 'A PDZ-Binding motif controls basolateral targeting of syndecan-1 along the biosynthetic pathway in polarized epithelial cells', Traffic, vol. 9, no. 11, pp. 1915-1924. https://doi.org/10.1111/j.1600-0854.2008.00805.x
Maday, Sandra ; Anderson, Eric ; Chang, Henry C. ; Shorter, James ; Satoh, Ayano ; Sfakianos, Jeff ; Fölsch, Heike ; Anderson, James M. ; Walther, Zenta ; Mellman, Ira. / A PDZ-Binding motif controls basolateral targeting of syndecan-1 along the biosynthetic pathway in polarized epithelial cells. In: Traffic. 2008 ; Vol. 9, No. 11. pp. 1915-1924.
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