A pathway from conventional to next generation methods to identify the sex determinant in persimmon

T. Akagi, R. Tao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cultivated Diospyros kaki, otherwise known as persimmon or kaki, is a polygamous species, whereas most other Diospyros species are dioecious. Most commercial cultivars of D. kaki bear only female flowers; however, some cultivars bear male flowers as well. Elucidation of the genetic and molecular basis of sex expression in D. kaki may lead to the development of artificial control of sex expression in this important fruit species. To this end, Diospyros lotus was used as a model to study the sexuality of D. kaki. Using a population of 63 F1 offspring of D. lotus, a bulked segregant analysis with amplified fragment length polymorphisms (AFLP) showed that the sexuality of this species is controlled by a single locus or haploblock in a heterogametic male (XY) system. Male-specific subsequences from Illumina HiSeq genomic reads data obtained from male and female pools were cataloged and assembled. This enabled comprehensive isolation of the male-determining regions on the Y-chromosome of D. lotus. Integration of transcriptome and evolutionary analyses led to the identification of one Y-specific sex-determinant candidate gene, OGI. This gene encodes a small RNA that triggers a transitive RNAi on the feminizing gene, MeGI, which is located on the autosome or the pseudoautosomal region of the sex chromosome. Molecular markers developed from the OGI sequence and its flanking regions indicated that the genetic control of maleness expression in D. kaki is based essentially on the OGI/MeGI system.

Original languageEnglish
Title of host publication6th International Symposium on Persimmon
EditorsM.M. Naval, M.L. Badenes
PublisherInternational Society for Horticultural Science
Pages71-74
Number of pages4
ISBN (Electronic)9789462611917
DOIs
Publication statusPublished - Mar 31 2018
Externally publishedYes

Publication series

NameActa Horticulturae
Volume1195
ISSN (Print)0567-7572

Fingerprint

Diospyros kaki
persimmons
Diospyros lotus
gender
Diospyros
methodology
male flowers
genes
female flowers
autosomes
sex chromosomes
Y chromosome
cultivars
transcriptome
amplified fragment length polymorphism
RNA
genomics
loci
genetic markers
fruits

Keywords

  • Amplified fragment polymorphisms
  • Diospyros
  • Next generation sequencing
  • Sex chromosome
  • Sexuality

ASJC Scopus subject areas

  • Horticulture

Cite this

Akagi, T., & Tao, R. (2018). A pathway from conventional to next generation methods to identify the sex determinant in persimmon. In M. M. Naval, & M. L. Badenes (Eds.), 6th International Symposium on Persimmon (pp. 71-74). (Acta Horticulturae; Vol. 1195). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2018.1195.12

A pathway from conventional to next generation methods to identify the sex determinant in persimmon. / Akagi, T.; Tao, R.

6th International Symposium on Persimmon. ed. / M.M. Naval; M.L. Badenes. International Society for Horticultural Science, 2018. p. 71-74 (Acta Horticulturae; Vol. 1195).

Research output: Chapter in Book/Report/Conference proceedingChapter

Akagi, T & Tao, R 2018, A pathway from conventional to next generation methods to identify the sex determinant in persimmon. in MM Naval & ML Badenes (eds), 6th International Symposium on Persimmon. Acta Horticulturae, vol. 1195, International Society for Horticultural Science, pp. 71-74. https://doi.org/10.17660/ActaHortic.2018.1195.12
Akagi T, Tao R. A pathway from conventional to next generation methods to identify the sex determinant in persimmon. In Naval MM, Badenes ML, editors, 6th International Symposium on Persimmon. International Society for Horticultural Science. 2018. p. 71-74. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2018.1195.12
Akagi, T. ; Tao, R. / A pathway from conventional to next generation methods to identify the sex determinant in persimmon. 6th International Symposium on Persimmon. editor / M.M. Naval ; M.L. Badenes. International Society for Horticultural Science, 2018. pp. 71-74 (Acta Horticulturae).
@inbook{c6245c48afe844d7af80b3f600712ef4,
title = "A pathway from conventional to next generation methods to identify the sex determinant in persimmon",
abstract = "Cultivated Diospyros kaki, otherwise known as persimmon or kaki, is a polygamous species, whereas most other Diospyros species are dioecious. Most commercial cultivars of D. kaki bear only female flowers; however, some cultivars bear male flowers as well. Elucidation of the genetic and molecular basis of sex expression in D. kaki may lead to the development of artificial control of sex expression in this important fruit species. To this end, Diospyros lotus was used as a model to study the sexuality of D. kaki. Using a population of 63 F1 offspring of D. lotus, a bulked segregant analysis with amplified fragment length polymorphisms (AFLP) showed that the sexuality of this species is controlled by a single locus or haploblock in a heterogametic male (XY) system. Male-specific subsequences from Illumina HiSeq genomic reads data obtained from male and female pools were cataloged and assembled. This enabled comprehensive isolation of the male-determining regions on the Y-chromosome of D. lotus. Integration of transcriptome and evolutionary analyses led to the identification of one Y-specific sex-determinant candidate gene, OGI. This gene encodes a small RNA that triggers a transitive RNAi on the feminizing gene, MeGI, which is located on the autosome or the pseudoautosomal region of the sex chromosome. Molecular markers developed from the OGI sequence and its flanking regions indicated that the genetic control of maleness expression in D. kaki is based essentially on the OGI/MeGI system.",
keywords = "Amplified fragment polymorphisms, Diospyros, Next generation sequencing, Sex chromosome, Sexuality",
author = "T. Akagi and R. Tao",
year = "2018",
month = "3",
day = "31",
doi = "10.17660/ActaHortic.2018.1195.12",
language = "English",
series = "Acta Horticulturae",
publisher = "International Society for Horticultural Science",
pages = "71--74",
editor = "M.M. Naval and M.L. Badenes",
booktitle = "6th International Symposium on Persimmon",

}

TY - CHAP

T1 - A pathway from conventional to next generation methods to identify the sex determinant in persimmon

AU - Akagi, T.

AU - Tao, R.

PY - 2018/3/31

Y1 - 2018/3/31

N2 - Cultivated Diospyros kaki, otherwise known as persimmon or kaki, is a polygamous species, whereas most other Diospyros species are dioecious. Most commercial cultivars of D. kaki bear only female flowers; however, some cultivars bear male flowers as well. Elucidation of the genetic and molecular basis of sex expression in D. kaki may lead to the development of artificial control of sex expression in this important fruit species. To this end, Diospyros lotus was used as a model to study the sexuality of D. kaki. Using a population of 63 F1 offspring of D. lotus, a bulked segregant analysis with amplified fragment length polymorphisms (AFLP) showed that the sexuality of this species is controlled by a single locus or haploblock in a heterogametic male (XY) system. Male-specific subsequences from Illumina HiSeq genomic reads data obtained from male and female pools were cataloged and assembled. This enabled comprehensive isolation of the male-determining regions on the Y-chromosome of D. lotus. Integration of transcriptome and evolutionary analyses led to the identification of one Y-specific sex-determinant candidate gene, OGI. This gene encodes a small RNA that triggers a transitive RNAi on the feminizing gene, MeGI, which is located on the autosome or the pseudoautosomal region of the sex chromosome. Molecular markers developed from the OGI sequence and its flanking regions indicated that the genetic control of maleness expression in D. kaki is based essentially on the OGI/MeGI system.

AB - Cultivated Diospyros kaki, otherwise known as persimmon or kaki, is a polygamous species, whereas most other Diospyros species are dioecious. Most commercial cultivars of D. kaki bear only female flowers; however, some cultivars bear male flowers as well. Elucidation of the genetic and molecular basis of sex expression in D. kaki may lead to the development of artificial control of sex expression in this important fruit species. To this end, Diospyros lotus was used as a model to study the sexuality of D. kaki. Using a population of 63 F1 offspring of D. lotus, a bulked segregant analysis with amplified fragment length polymorphisms (AFLP) showed that the sexuality of this species is controlled by a single locus or haploblock in a heterogametic male (XY) system. Male-specific subsequences from Illumina HiSeq genomic reads data obtained from male and female pools were cataloged and assembled. This enabled comprehensive isolation of the male-determining regions on the Y-chromosome of D. lotus. Integration of transcriptome and evolutionary analyses led to the identification of one Y-specific sex-determinant candidate gene, OGI. This gene encodes a small RNA that triggers a transitive RNAi on the feminizing gene, MeGI, which is located on the autosome or the pseudoautosomal region of the sex chromosome. Molecular markers developed from the OGI sequence and its flanking regions indicated that the genetic control of maleness expression in D. kaki is based essentially on the OGI/MeGI system.

KW - Amplified fragment polymorphisms

KW - Diospyros

KW - Next generation sequencing

KW - Sex chromosome

KW - Sexuality

UR - http://www.scopus.com/inward/record.url?scp=85046710798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046710798&partnerID=8YFLogxK

U2 - 10.17660/ActaHortic.2018.1195.12

DO - 10.17660/ActaHortic.2018.1195.12

M3 - Chapter

AN - SCOPUS:85046710798

T3 - Acta Horticulturae

SP - 71

EP - 74

BT - 6th International Symposium on Persimmon

A2 - Naval, M.M.

A2 - Badenes, M.L.

PB - International Society for Horticultural Science

ER -