A novel WD40 repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner

Sachio Ito, Akiko Sakai, Takako Nomura, Yukari Miki, Mamoru Ouchida, Junzo Sasaki, Kenji Shimizu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have cloned a novel cDNA encoding a protein with eight WD repeat motifs and a domain similar to collagen. As the predicted size of the protein was 146 kDa, the gene was named WDC146. Here, we characterized the genomic structure, gene products, and the expression profiles. The human WDC146 gene had 22 exons spanning over 105 kb, and these exons were distributed in three islands intervened by two long introns of around 40 kb. A minimum promoter region was identified within a 0.5 kb 5′-upstream region of exon 1. WDC146 mRNA was most highly expressed in human testis on Northern blot analysis. In mouse tissues, the highest expression was also observed in testis. By in situ hybridization on rat tissues, WDC146 mRNA was detected preferentially in the pachytene stage of spermatocytes in testis, and weakly in white pulp/marginal band of spleen and in cortex of thymus. WDC146 protein was found to be localized in nucleus. These data implied that WDC146 protein may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination.

Original languageEnglish
Pages (from-to)656-663
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number3
DOIs
Publication statusPublished - Jan 2001

Keywords

  • Collagen-like domain
  • Meiosis
  • Promoter
  • Spermatogenesis
  • WD40-repeat

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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