A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering

Tetsuro Mazaki, Yasuyuki Shiozaki, Kentaro Yamane, Aki Yoshida, Mariko Nakamura, Yasuhiro Yoshida, Di Zhou, Takashi Kitajima, Masato Tanaka, Yoshihiro Ito, Toshihumi Ozaki, Akihiro Matsukawa

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37°C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.

Original languageEnglish
Article number4457
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - Mar 25 2014

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Gelatin
Tissue Engineering
Light
Mesenchymal Stromal Cells
Rose Bengal
Collagen
Hydrogels
Hydrogel
Wounds and Injuries
Articular Cartilage
Rabbits
Bone and Bones
Proteins

ASJC Scopus subject areas

  • General

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A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering. / Mazaki, Tetsuro; Shiozaki, Yasuyuki; Yamane, Kentaro; Yoshida, Aki; Nakamura, Mariko; Yoshida, Yasuhiro; Zhou, Di; Kitajima, Takashi; Tanaka, Masato; Ito, Yoshihiro; Ozaki, Toshihumi; Matsukawa, Akihiro.

In: Scientific Reports, Vol. 4, 4457, 25.03.2014.

Research output: Contribution to journalArticle

Mazaki, Tetsuro ; Shiozaki, Yasuyuki ; Yamane, Kentaro ; Yoshida, Aki ; Nakamura, Mariko ; Yoshida, Yasuhiro ; Zhou, Di ; Kitajima, Takashi ; Tanaka, Masato ; Ito, Yoshihiro ; Ozaki, Toshihumi ; Matsukawa, Akihiro. / A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering. In: Scientific Reports. 2014 ; Vol. 4.
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