A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum

Shuangchao Wang, Hideki Kondo, Liang Liu, Lihua Guo, Dewen Qiu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

•We isolated a novel mycovirus from the plant pathogenic fungus F. graminearum.•We sequenced the full-length of the virus with a genome of 13,023 nucleotides.•Phylogenetic analysis suggested FgHV1 was closely related to CHV1 and CHV2.•FgHV1 infection resulted in no dramatic phenotypic alteration to the host fungus. A double-stranded (ds) RNA element, sized at approximately 13. kb pairs, was purified from a field isolate, HN10, of Fusarium graminearum. The coding strand of the dsRNA was 13,023 nucleotides (nt) long (excluding the 3' poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORF A and ORF B). The 5' proximal ORF A of 531. nt encoded a protein of 176 amino acids (aa), and a BLAST search showed it to be similar to the putative papain-like protease domains encoded by Valsa ceratosperma hypovirus 1 (35% identity) and Cryphonectria hypovirus 4 (CHV4) (31% identity). The 3' proximal ORF B of 11,118. nt encoded a large polyprotein with three conserved domains, including papain-like protease, RNA-dependent RNA polymerase and RNA helicase domains. The polyprotein shared significant aa identities with CHV1 (32%) and CHV2 (32%). Both the genome organization and phylogenetic analysis suggested that the characterized RNA represented a novel hypovirus, designated "Fusarium graminearum hypovirus 1 (FgHV1)", which was closely related to CHV1 and CHV2 in the Hypoviridae family. Elimination of the virus resulted in no dramatic phenotypic alteration of the fungus.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalVirus Research
Volume174
Issue number1-2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Fusarium
Open Reading Frames
Fungi
Viruses
Nucleotides
Polyproteins
Papain
Peptide Hydrolases
Genome
RNA Helicases
RNA Replicase
Amino Acids
Double-Stranded RNA
RNA
Messenger RNA
Infection
Proteins

Keywords

  • DsRNA
  • FgHV1
  • Fusarium graminearum
  • Fusarium head blight
  • Hypoviridae
  • Mycovirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. / Wang, Shuangchao; Kondo, Hideki; Liu, Liang; Guo, Lihua; Qiu, Dewen.

In: Virus Research, Vol. 174, No. 1-2, 06.2013, p. 69-77.

Research output: Contribution to journalArticle

Wang, Shuangchao ; Kondo, Hideki ; Liu, Liang ; Guo, Lihua ; Qiu, Dewen. / A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. In: Virus Research. 2013 ; Vol. 174, No. 1-2. pp. 69-77.
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