A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase

M. Osawa, Hiroshi Tokumitsu, M. B. Swindells, H. Kurihara, M. Orita, T. Shibanuma, T. Furuya, M. Ikura

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

The structure of calcium-bound calmodulin (Ca2+/CaM) complexed with a 26-residue peptide, corresponding to the CaM-binding domain of rat Ca2+/CaM-dependent protein kinase kinase (CaMKK), has been determined by NMR spectroscopy. In this complex, the CaMKK peptide forms a fold comprising an α-helix and a hairpin-like loop whose C-terminus folds back on itself. The binding orientation of this CaMKK peptide by the two CaM domains is opposite to that observed in all other CaM-target complexes determined so far. The N- and C-terminal hydrophobic pockets of Ca2+/CaM anchor Trp 444 and Phe 459 of the CaMKK peptide, respectively. This 14-residue separation between two key hydrophobic groups is also unique among previously determined CaM complexes. The present structure represents a new and distinct class of Ca2+/CaM target recognition that may be shared by other Ca2+/CaM- stimulated proteins.

Original languageEnglish
Pages (from-to)819-824
Number of pages6
JournalNature Structural Biology
Volume6
Issue number9
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Protein Kinases
Phosphotransferases
Peptides
Anchors
Nuclear magnetic resonance spectroscopy
Rats
Magnetic Resonance Spectroscopy
Calcium
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase. / Osawa, M.; Tokumitsu, Hiroshi; Swindells, M. B.; Kurihara, H.; Orita, M.; Shibanuma, T.; Furuya, T.; Ikura, M.

In: Nature Structural Biology, Vol. 6, No. 9, 1999, p. 819-824.

Research output: Contribution to journalArticle

Osawa, M, Tokumitsu, H, Swindells, MB, Kurihara, H, Orita, M, Shibanuma, T, Furuya, T & Ikura, M 1999, 'A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase', Nature Structural Biology, vol. 6, no. 9, pp. 819-824. https://doi.org/10.1038/12271
Osawa, M. ; Tokumitsu, Hiroshi ; Swindells, M. B. ; Kurihara, H. ; Orita, M. ; Shibanuma, T. ; Furuya, T. ; Ikura, M. / A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase. In: Nature Structural Biology. 1999 ; Vol. 6, No. 9. pp. 819-824.
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