A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure

N. Okada, K. Mizuta, M. Oshima, N. Yamada, Y. Sanada, Y. Ihara, T. Urahashi, J. Ishikawa, T. Tsuji, S. Hishikawa, T. Teratani, E. Kobayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background A merit of subnormothermic perfusion has been reported to preserve grafts from ischemic injury in animal models. The split liver technique is commonly performed to solve the shortage of liver grafts. However, there has been no study showing the effect of a split liver graft on subnormothermic perfusion. We herein investigated the split liver protocol using a subnormothermic oxygenated circuit system (SOCS). Methods Auxiliary liver transplantation was performed in a porcine marginal donor model by using a SOCS. In the SOCS group, the portal vein and hepatic artery of the graft were cannulated, and the graft was perfused by SOCS. In the cold storage (CS) group, the graft was placed in cold preservation solution. In the preservation phase, the graft was split. Results There were no significant differences in the biochemical markers between the SOCS and CS groups. In terms of the histology, the sinusoidal spaces were widened in the CS group 12 hours after implantation. Conclusion We have demonstrated a possibility to use SOCS with the split liver protocol by using a porcine model. This split liver protocol using SOCS will extend the split liver criteria and rescue more patients from hepatic failure, including pediatric patients.

Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalTransplantation Proceedings
Volume47
Issue number2
DOIs
Publication statusPublished - Mar 1 2015
Externally publishedYes

Fingerprint

Swine
Tissue Donors
Transplants
Liver
Perfusion
Hepatic Artery
Liver Failure
Portal Vein
Liver Transplantation
Histology
Animal Models
Biomarkers
Pediatrics
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure. / Okada, N.; Mizuta, K.; Oshima, M.; Yamada, N.; Sanada, Y.; Ihara, Y.; Urahashi, T.; Ishikawa, J.; Tsuji, T.; Hishikawa, S.; Teratani, T.; Kobayashi, E.

In: Transplantation Proceedings, Vol. 47, No. 2, 01.03.2015, p. 419-426.

Research output: Contribution to journalArticle

Okada, N, Mizuta, K, Oshima, M, Yamada, N, Sanada, Y, Ihara, Y, Urahashi, T, Ishikawa, J, Tsuji, T, Hishikawa, S, Teratani, T & Kobayashi, E 2015, 'A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure', Transplantation Proceedings, vol. 47, no. 2, pp. 419-426. https://doi.org/10.1016/j.transproceed.2014.10.053
Okada, N. ; Mizuta, K. ; Oshima, M. ; Yamada, N. ; Sanada, Y. ; Ihara, Y. ; Urahashi, T. ; Ishikawa, J. ; Tsuji, T. ; Hishikawa, S. ; Teratani, T. ; Kobayashi, E. / A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure. In: Transplantation Proceedings. 2015 ; Vol. 47, No. 2. pp. 419-426.
@article{7c082dfd4196426fa088b86c81fe26c3,
title = "A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure",
abstract = "Background A merit of subnormothermic perfusion has been reported to preserve grafts from ischemic injury in animal models. The split liver technique is commonly performed to solve the shortage of liver grafts. However, there has been no study showing the effect of a split liver graft on subnormothermic perfusion. We herein investigated the split liver protocol using a subnormothermic oxygenated circuit system (SOCS). Methods Auxiliary liver transplantation was performed in a porcine marginal donor model by using a SOCS. In the SOCS group, the portal vein and hepatic artery of the graft were cannulated, and the graft was perfused by SOCS. In the cold storage (CS) group, the graft was placed in cold preservation solution. In the preservation phase, the graft was split. Results There were no significant differences in the biochemical markers between the SOCS and CS groups. In terms of the histology, the sinusoidal spaces were widened in the CS group 12 hours after implantation. Conclusion We have demonstrated a possibility to use SOCS with the split liver protocol by using a porcine model. This split liver protocol using SOCS will extend the split liver criteria and rescue more patients from hepatic failure, including pediatric patients.",
author = "N. Okada and K. Mizuta and M. Oshima and N. Yamada and Y. Sanada and Y. Ihara and T. Urahashi and J. Ishikawa and T. Tsuji and S. Hishikawa and T. Teratani and E. Kobayashi",
year = "2015",
month = "3",
day = "1",
doi = "10.1016/j.transproceed.2014.10.053",
language = "English",
volume = "47",
pages = "419--426",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "2",

}

TY - JOUR

T1 - A novel split liver protocol using the subnormothermic oxygenated circuit system in a porcine model of a marginal donor procedure

AU - Okada, N.

AU - Mizuta, K.

AU - Oshima, M.

AU - Yamada, N.

AU - Sanada, Y.

AU - Ihara, Y.

AU - Urahashi, T.

AU - Ishikawa, J.

AU - Tsuji, T.

AU - Hishikawa, S.

AU - Teratani, T.

AU - Kobayashi, E.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Background A merit of subnormothermic perfusion has been reported to preserve grafts from ischemic injury in animal models. The split liver technique is commonly performed to solve the shortage of liver grafts. However, there has been no study showing the effect of a split liver graft on subnormothermic perfusion. We herein investigated the split liver protocol using a subnormothermic oxygenated circuit system (SOCS). Methods Auxiliary liver transplantation was performed in a porcine marginal donor model by using a SOCS. In the SOCS group, the portal vein and hepatic artery of the graft were cannulated, and the graft was perfused by SOCS. In the cold storage (CS) group, the graft was placed in cold preservation solution. In the preservation phase, the graft was split. Results There were no significant differences in the biochemical markers between the SOCS and CS groups. In terms of the histology, the sinusoidal spaces were widened in the CS group 12 hours after implantation. Conclusion We have demonstrated a possibility to use SOCS with the split liver protocol by using a porcine model. This split liver protocol using SOCS will extend the split liver criteria and rescue more patients from hepatic failure, including pediatric patients.

AB - Background A merit of subnormothermic perfusion has been reported to preserve grafts from ischemic injury in animal models. The split liver technique is commonly performed to solve the shortage of liver grafts. However, there has been no study showing the effect of a split liver graft on subnormothermic perfusion. We herein investigated the split liver protocol using a subnormothermic oxygenated circuit system (SOCS). Methods Auxiliary liver transplantation was performed in a porcine marginal donor model by using a SOCS. In the SOCS group, the portal vein and hepatic artery of the graft were cannulated, and the graft was perfused by SOCS. In the cold storage (CS) group, the graft was placed in cold preservation solution. In the preservation phase, the graft was split. Results There were no significant differences in the biochemical markers between the SOCS and CS groups. In terms of the histology, the sinusoidal spaces were widened in the CS group 12 hours after implantation. Conclusion We have demonstrated a possibility to use SOCS with the split liver protocol by using a porcine model. This split liver protocol using SOCS will extend the split liver criteria and rescue more patients from hepatic failure, including pediatric patients.

UR - http://www.scopus.com/inward/record.url?scp=84926159329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926159329&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2014.10.053

DO - 10.1016/j.transproceed.2014.10.053

M3 - Article

C2 - 25769584

AN - SCOPUS:84926159329

VL - 47

SP - 419

EP - 426

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 2

ER -