A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses

Rui Zhang, Shengxue Liu, Sotaro Chiba, Hideki Kondo, Satoko Kanematsu, Nobuhiro Suzuki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Here we report a biological and molecular characterization of a novel positive-sense RNA virus isolated from a field isolate (NW10) of a filamentous phytopathogenic fungus, the white root rot fungus that is designated as Rosellinia necatrix fusarivirus 1(RnFV1). A recently developed technology using zinc ions allowed us to transfer RnFV1 to two mycelially incompatible Rosellinia necatrix strains. A biological comparison of the virus-free and -recipient isogenic fungal strains suggested that RnFV1 infects latently and thus has no potential as a virocontrol agent. The virus has an undivided positive-sense RNA genome of 6286 nucleotides excluding a poly (A) tail. The genome possesses two non-overlapping open reading frames (ORFs): a large ORF1 that encodes polypeptides with RNA replication functions and a smaller ORF2 that encodes polypeptides of unknown function. A lack of coat protein genes was suggested by the failure of virus particles from infected mycelia. No evidence was obtained by Northern analysis or classical 5′-RACE for the presence of subgenomic RNA for the downstream ORF. Sequence similarities were found in amino-acid sequence between RnFV1 putative proteins and counterparts of a previously reported mycovirus, Fusarium graminearum virus 1 (FgV1). Interestingly, several related sequences were detected by BLAST searches of independent transcriptome assembly databases one of which probably represents an entire virus genome. Phylogenetic analysis based on the conserved RNA-dependent RNA polymerase showed that RnFV1, FgV1, and these similar sequences are grouped in a cluster distinct from distantly related hypoviruses. It is proposed that a new taxonomic family termed Fusariviridae be created to include RnFV1 and FgV1.

Original languageEnglish
JournalFrontiers in Microbiology
Volume5
Issue numberJULY
DOIs
Publication statusPublished - 2014

Fingerprint

RNA Viruses
Fungi
Viruses
Fusarium
Genome
RNA
Open Reading Frames
RNA Replicase
Peptides
Mycelium
Capsid Proteins
Transcriptome
Virion
Zinc
Amino Acid Sequence
Nucleotides
Databases
Ions
Technology
Messenger RNA

Keywords

  • Fusarivurs
  • Hypovirus
  • Novel mycovirus
  • Rosellinia necatrix
  • ssRNA virus
  • Transcriptome shotgun assembly

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses. / Zhang, Rui; Liu, Shengxue; Chiba, Sotaro; Kondo, Hideki; Kanematsu, Satoko; Suzuki, Nobuhiro.

In: Frontiers in Microbiology, Vol. 5, No. JULY, 2014.

Research output: Contribution to journalArticle

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