A novel short-root gene that affects specifically early root development in rice (Oryza sativa L.)

Shan Guo Yao, Shin Taketa, Masahiko Ichii

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have isolated a novel short-root mutant, srt5, from an M2 population of rice (Oryza sativa L. cv. Oochikara) treated with NaN3, and mapped its causal gene to chromosome 2. The mutant showed extreme inhibition of seminal root, crown root and lateral root elongation, and altered root hair formation at the seedling stage. Histological observation of seminal root tips indicated that the short-root phenotype was due to the reduced cell size and cell number. At later stages of plant development, roots of the mutant elongated acceleratingly and recovered normal morphology 45 days after transplantation, and adult plants of the mutant resembled the wild type except for reduced pollen and seed fertilities. Seminal root growth of the mutant was partially rescued by exogenously applied ABA, suggesting that ABA biosynthesis might be affected in srt5 plants. Moreover, the mutant also showed high resistance to 2,4-D, GA3 and KIN based on seminal root growth assays, but the resistance to these hormones disappeared when ABA was concomitantly applied. Thus, the wild type allele of srt5 seems to be of great importance for early root development by altering plant hormone homeostasis, in which ABA plays a critical role.

Original languageEnglish
Pages (from-to)207-215
Number of pages9
JournalPlant Science
Volume163
Issue number2
DOIs
Publication statusPublished - Aug 2002
Externally publishedYes

Fingerprint

Oryza sativa
Genes
rice
Sodium Azide
mutants
2,4-Dichlorophenoxyacetic Acid
Plant Roots
Plant Growth Regulators
Chromosomes, Human, Pair 2
Plant Development
Meristem
abscisic acid
Biosynthesis
Chromosomes
Growth
Pollen
Crowns
Seedlings
Cell Size
Fertility

Keywords

  • ABA
  • Hormone response
  • Morphology
  • Mutant
  • Rice
  • Root

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

A novel short-root gene that affects specifically early root development in rice (Oryza sativa L.). / Yao, Shan Guo; Taketa, Shin; Ichii, Masahiko.

In: Plant Science, Vol. 163, No. 2, 08.2002, p. 207-215.

Research output: Contribution to journalArticle

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