A Novel Role of Nectins in Inhibition of the E-Cadherin-induced Activation of Rac and Formation of Cell-Cell Adherens Junctions

Takashi Hoshino, Kazuya Shimizu, Tomoyuki Honda, Tomomi Kawakatsu, Taihei Fukuyama, Takeshi Nakamura, Michiyuki Matsuda, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Nectins are Ca2+-independent immunoglobulin (Ig)-like cell-cell adhesion molecules. The trans-interactions of nectins recruit cadherins to the nectin-based cell-cell adhesion, resulting in formation of cell-cell adherens junctions (AJs) in epithelial cells and fibroblasts. The trans-interaction of E-cadherin induces activation of Rac small G protein, whereas the trans-interactions of nectins induce activation of not only Rac but also Cdc42 small G protein. We showed by the fluorescent resonance energy transfer (FRET) imaging that the trans-interaction of E-cadherin induced dynamic activation and inactivation of Rac, which led to dynamic formation and retraction of lamellipodia. Moreover, we found here that the nectins, which did not trans-interact with other nectins (non-trans-interacting nectins), inhibited the E-cadherin-induced activation of Rac and reduced the velocity of the formation of the E-cadherin-based cell-cell AJs. The inhibitory effect of non-trans-interacting nectins was suppressed by the activation of Cdc42 induced by the trans-interactions of nectins. These results indicate a novel role of nectins in regulation of the E-cadherin-induced activation of Rac and formation of cell-cell AJs.

Original languageEnglish
Pages (from-to)1077-1088
Number of pages12
JournalMolecular Biology of the Cell
Volume15
Issue number3
DOIs
Publication statusPublished - Mar 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'A Novel Role of Nectins in Inhibition of the E-Cadherin-induced Activation of Rac and Formation of Cell-Cell Adherens Junctions'. Together they form a unique fingerprint.

Cite this