A novel role for Rad17 in homologous recombination

Katsuaki Nishino, Eri Inoue, Shunya Takada, Takuya Abe, Motomu Akita, Akari Yoshimura, Shusuke Tada, Masahiko Kobayashi, Ken Ichi Yamamoto, Masayuki Seki, Takemi Enomoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Replication checkpoint protein Radl7 senses DNA lesions during DNA replication and halts progression of replication fork. The cells derived from Bloom syndrome individuals show some defects in DNA replication. In order to investigate the functional relationship between the replication checkpoint protein Radl7 and BLM, which is the product of the causative gene of Bloom syndrome, we generated BLM/RAD17 double knockout (blm/rad17) cells using chicken DT40 cells. The blm/rad17 cells showed exaggerated growth defects as determined by analysis of their growth curves and plating efficiency compared to those of either of the single gene mutants. These defects seem to be due to an increase in DNA lesions that cause spontaneous cell death, suggesting that Rad17 and BLM execute different functions in the progression of replication forks. We also demonstrate that targeting integration was dramatically compromised by a lack of Rad17. In addition, the elevated frequency of sister chromatid exchange (SCE) due to homologous recombination in BLM knockout (blm) cells was greatly reduced by disruption of the RAD17 gene. Thus, in addition to its role in the replication checkpoint, Rad17 appears to play a role in homologous recombination.

Original languageEnglish
Pages (from-to)427-431
Number of pages5
JournalGenes and Genetic Systems
Volume83
Issue number5
DOIs
Publication statusPublished - Dec 1 2008
Externally publishedYes

Keywords

  • ATR
  • DT40
  • RAD17
  • RecQ
  • Replication checkpoint

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Nishino, K., Inoue, E., Takada, S., Abe, T., Akita, M., Yoshimura, A., Tada, S., Kobayashi, M., Yamamoto, K. I., Seki, M., & Enomoto, T. (2008). A novel role for Rad17 in homologous recombination. Genes and Genetic Systems, 83(5), 427-431. https://doi.org/10.1266/ggs.83.427