A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions

Kiyotaka Nagaki, Hisashi Tsujimoto, Tetsuo Sasakuma

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 x 105 per haploid sugar cane genome and, therefore, 4.6 x 103 or 630 kb per chromosome on average.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalChromosome Research
Volume6
Issue number4
DOIs
Publication statusPublished - Jul 29 1998
Externally publishedYes

Keywords

  • CENP-B box
  • FISH
  • Saccharum species
  • Tandem repeat

ASJC Scopus subject areas

  • Genetics

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