A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions

Kiyotaka Nagaki; Hisashi Tsujimoto; Tetsuo Sasakuma

doi:10.1023/A:1009270824142

A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions

Kiyotaka Nagaki, Hisashi Tsujimoto, Tetsuo Sasakuma

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 x 10⁵ per haploid sugar cane genome and, therefore, 4.6 x 10³ or 630 kb per chromosome on average.

Original language	English
Pages (from-to)	295-302
Number of pages	8
Journal	Chromosome Research
Volume	6
Issue number	4
DOIs	https://doi.org/10.1023/A:1009270824142
Publication status	Published - 1998
Externally published	Yes

Keywords

CENP-B box
FISH
Saccharum species
Tandem repeat

ASJC Scopus subject areas

Genetics

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title = "A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions",

abstract = "Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 x 105 per haploid sugar cane genome and, therefore, 4.6 x 103 or 630 kb per chromosome on average.",

keywords = "CENP-B box, FISH, Saccharum species, Tandem repeat",

author = "Kiyotaka Nagaki and Hisashi Tsujimoto and Tetsuo Sasakuma",

note = "Funding Information: This work was supported by a research fellowship (No. 5924) from the Japan Society for the Promotion of Science for Young Scientists.",

year = "1998",

doi = "10.1023/A:1009270824142",

language = "English",

volume = "6",

pages = "295--302",

journal = "Chromosome Research",

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T1 - A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions

AU - Nagaki, Kiyotaka

AU - Tsujimoto, Hisashi

AU - Sasakuma, Tetsuo

N1 - Funding Information: This work was supported by a research fellowship (No. 5924) from the Japan Society for the Promotion of Science for Young Scientists.

PY - 1998

Y1 - 1998

N2 - Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 x 105 per haploid sugar cane genome and, therefore, 4.6 x 103 or 630 kb per chromosome on average.

AB - Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 x 105 per haploid sugar cane genome and, therefore, 4.6 x 103 or 630 kb per chromosome on average.

KW - CENP-B box

KW - FISH

KW - Saccharum species

KW - Tandem repeat

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