A novel real-time DNA detection system for loop-mediated isothermal amplification method

Koji Kakugawa, Kenji Yamada, Hiroshi Maeda, Shogo Takashiba

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1 Citation (Scopus)

Abstract

We developed a novel real-time DNA detection system for loop-mediated isothermal amplification (LAMP) method. Our prototype was composed of a thermostatic chamber, a hole slide glass, LED and a web camera. The reaction mixture was injected into the slide glass hole and the LAMP reaction was carried out at 63°C for 2 hours. To observe the DNA amplification, we monitored the fluorescence intensity of SYBR Green I that was excited by the blue LED. The captured BMP images were analyzed by NIH Image J software. The DNA amplification and amplification monitoring experiment was successful. Furthermore, quantitative accuracy was evaluated based on real-time PCR. The reaction time correlates well with the DNA concentration. These results indicate the successful development of a novel real-time DNA detection system for LAMP method.

Original languageEnglish
JournalIEEJ Transactions on Electronics, Information and Systems
Volume130
Issue number5
DOIs
Publication statusPublished - 2010

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Keywords

  • Aspiration pneumonia
  • DNA
  • Lamp method
  • Oral care
  • Periodontal pathogen

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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