A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco

Ivan Galis, Petr Šimek, Tomoko Narisawa, Mami Sasaki, Tatsuya Horiguchi, Hiroo Fukuda, Ken Matsuoka

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Target metabolic and large-scale transcriptomic analyses of tobacco (Nicotiana tabacum L.) Bright Yellow-2 (BY-2) cells were employed to identify novel gene(s) involved in methyl jasmonate (MJ)-dependent function in plants. At the metabolic level, we describe the specific accumulation of several phenylpropanoid-polyamine conjugates in MJ-treated BY-2 cells. Furthermore, global gene expression analysis of MJ-treated cells using a 16K cDNA microarray containing expressed sequence tags (ESTs) from BY-2 cells revealed 828 genes that were upregulated by MJ treatment within 48 h. Using time-course expression data we identified a novel MJ-inducible R2R3 MYB-type transcription factor (NtMYBJS1) that was co-expressed in a close temporal pattern with the core phenylpropanoid genes phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). Overexpression of NtMYBJS1 in tobacco BY-2 cells caused accumulation of specific phenylpropanoid conjugates in the cells. Subsequent microarray analysis of NtMYBJS1 transgenic lines revealed that a limited number of genes, including PAL and 4CL, were specifically induced in the presence of the NtMYBJS1 transgene. These results, together with results of both antisense expression analysis and of gel mobility shift assays, strongly indicate that the NtMYBJS1 protein functions in tobacco MJ signal transduction, inducing phenylpropanoid biosynthetic genes and the accumulation of phenylpropanoid-polyamine conjugates during stress.

Original languageEnglish
Pages (from-to)573-592
Number of pages20
JournalPlant Journal
Volume46
Issue number4
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Fingerprint

methyl jasmonate
phenylpropanoids
Tobacco
tobacco
Transcription Factors
transcription factors
biosynthesis
coumarate-CoA ligase
Phenylalanine Ammonia-Lyase
Genes
Polyamines
phenylalanine ammonia-lyase
cells
polyamines
genes
Expressed Sequence Tags
Electrophoretic Mobility Shift Assay
Microarray Analysis
Oligonucleotide Array Sequence Analysis
expressed sequence tags

Keywords

  • Methyl jasmonate
  • Microarray
  • MYB transcription factor
  • Natural bioproducts
  • Phenylpropanoid pathway
  • Tobacco BY-2 cells

ASJC Scopus subject areas

  • Plant Science

Cite this

A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco. / Galis, Ivan; Šimek, Petr; Narisawa, Tomoko; Sasaki, Mami; Horiguchi, Tatsuya; Fukuda, Hiroo; Matsuoka, Ken.

In: Plant Journal, Vol. 46, No. 4, 05.2006, p. 573-592.

Research output: Contribution to journalArticle

Galis, Ivan ; Šimek, Petr ; Narisawa, Tomoko ; Sasaki, Mami ; Horiguchi, Tatsuya ; Fukuda, Hiroo ; Matsuoka, Ken. / A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco. In: Plant Journal. 2006 ; Vol. 46, No. 4. pp. 573-592.
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AU - Matsuoka, Ken

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