A novel quantitative receptor autoradiography and in situ hybridization histochemistry technique using storage phosphor screen imaging

T. Ito, T. Suzuki, D. K. Lim, S. E. Wellman, I. K. Ho

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A new technique of image acquisition for quantitative receptor autoradiography and in situ hybridization histochemistry was developed using storage phosphor screen imaging. This method was at least 4-5 times faster than conventional film densitometry. Two of the advantages of the phosphor screen method are high sensitivity and wide linear range of response. Other aspects of this method were compared with those of conventional densitometry. Use of storage phosphor screen imaging will allow greatly increased speed of pharmacological screening procedures that utilize quantitative autoradiography.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalJournal of Neuroscience Methods
Volume59
Issue number2
DOIs
Publication statusPublished - Jul 1995
Externally publishedYes

Keywords

  • Densitometry
  • Digital imaging
  • In situ hybridization histochemistry
  • Receptor autoradiography
  • Storage phosphor screen
  • [S]t-Butylbicyclophosphorothionate

ASJC Scopus subject areas

  • Neuroscience(all)

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