A novel protein in Photosystem II of a diatom Chaetoceros gracilis is one of the extrinsic proteins located on lumenal side and directly associates with PSII core components

Akinori Okumura, Ryo Nagao, Takehiro Suzuki, Satoshi Yamagoe, Masako Iwai, Katsuyoshi Nakazato, Isao Enami

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The gene encoding a novel extrinsic protein (Psb31) found in Photosystem II (PSII) of a diatom, Chaetoceros gracilis, was cloned and sequenced. The deduced protein contained three characteristic leader sequences targeted for chloroplast endoplasmic reticulum membrane, chloroplast envelope membrane and thylakoid membrane, indicating that Psb31 is encoded in the nuclear genome and constitutes one of the extrinsic proteins located on the lumenal side. Homologous genes were found in a red alga and chromophytic algae but not in other organisms. Genes encoding the other four extrinsic proteins in C. gracilis PSII were also cloned and sequenced, and their leader sequences were characterized and compared. To search for the nearest neighbor relationship between Psb31 and the other PSII components, we crosslinked the PSII particles with the water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, and found that Psb31 directly associates with PSII core components through electrostatic interaction, suggesting that the novel Psb31 protein is one of the extrinsic proteins constituting the functional oxygen-evolving complex of C. gracilis PSII.

Original languageEnglish
Pages (from-to)1545-1551
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1777
Issue number12
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Fingerprint

Diatoms
Photosystem II Protein Complex
Proteins
Gene encoding
Chloroplasts
Algae
Membranes
Genes
Ethyldimethylaminopropyl Carbodiimide
Rhodophyta
Carbodiimides
Thylakoids
Protein C
Coulomb interactions
Static Electricity
Endoplasmic Reticulum
Genome
Oxygen
Water

Keywords

  • Chaetoceros gracilis
  • Diatom
  • Extrinsic protein
  • Oxygen evolution
  • Photosystem II
  • Psb31

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

A novel protein in Photosystem II of a diatom Chaetoceros gracilis is one of the extrinsic proteins located on lumenal side and directly associates with PSII core components. / Okumura, Akinori; Nagao, Ryo; Suzuki, Takehiro; Yamagoe, Satoshi; Iwai, Masako; Nakazato, Katsuyoshi; Enami, Isao.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1777, No. 12, 12.2008, p. 1545-1551.

Research output: Contribution to journalArticle

Okumura, Akinori ; Nagao, Ryo ; Suzuki, Takehiro ; Yamagoe, Satoshi ; Iwai, Masako ; Nakazato, Katsuyoshi ; Enami, Isao. / A novel protein in Photosystem II of a diatom Chaetoceros gracilis is one of the extrinsic proteins located on lumenal side and directly associates with PSII core components. In: Biochimica et Biophysica Acta - Bioenergetics. 2008 ; Vol. 1777, No. 12. pp. 1545-1551.
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