A novel photic entrainment mechanism for the circadian clock in an insect: Involvement of c-fos and cryptochromes

Yuki Kutaragi, Atsushi Tokuoka, Yasuaki Tomiyama, Motoki Nose, Takayuki Watanabe, Tetsuya Bando, Yoshiyuki Moriyama, Kenji Tomioka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Entrainment to the environmental light cycle is an essential property of the circadian clock. Although the compound eye is known to be the major photoreceptor necessary for entrainment in many insects, the molecular mechanisms of photic entrainment remain to be explored. Results: We found that cryptochromes (crys) and c-fos mediate photic entrainment of the circadian clock in a hemimetabolous insect, the cricket Gryllus bimaculatus. We examined the effects of RNA interference (RNAi)-mediated knockdown of the cry genes, Gb'cry1 and Gb'cry2, on photic entrainment, and light-induced resetting of the circadian locomotor rhythm. Gb'cry2 RNAi accelerated entrainment for delay shifts, while Gb'cry1/ Gb'cry2 double RNAi resulted in significant lengthening of transient cycles in both advance and delay shifts, and even in entrainment failure in some crickets. Double RNAi also strongly suppressed light induced resetting. The Gb'cry-mediated phase shift or resetting of the rhythm was preceded by light-induced Gb'c-fosB expression. We also found that Gb'c-fosB, Gb'cry2 and Gb'period (Gb'per) were likely co-expressed in some optic lobe neurons. Conclusion: Based on these results, we propose a novel model for photic entrainment of the insect circadian clock, which relies on the light information perceived by the compound eye.

Original languageEnglish
Article number26
JournalZoological Letters
Volume4
Issue number1
DOIs
Publication statusPublished - Sep 18 2018

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RNA interference
circadian rhythm
insects
compound eyes
Gryllidae
optic lobe
Gryllus bimaculatus
photoreceptors
photoperiod
neurons
cryptochromes
genes

Keywords

  • C-fos
  • Circadian clock
  • Clock gene
  • Cryptochrome
  • Insect
  • Photic entrainment

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

A novel photic entrainment mechanism for the circadian clock in an insect : Involvement of c-fos and cryptochromes. / Kutaragi, Yuki; Tokuoka, Atsushi; Tomiyama, Yasuaki; Nose, Motoki; Watanabe, Takayuki; Bando, Tetsuya; Moriyama, Yoshiyuki; Tomioka, Kenji.

In: Zoological Letters, Vol. 4, No. 1, 26, 18.09.2018.

Research output: Contribution to journalArticle

Kutaragi, Yuki ; Tokuoka, Atsushi ; Tomiyama, Yasuaki ; Nose, Motoki ; Watanabe, Takayuki ; Bando, Tetsuya ; Moriyama, Yoshiyuki ; Tomioka, Kenji. / A novel photic entrainment mechanism for the circadian clock in an insect : Involvement of c-fos and cryptochromes. In: Zoological Letters. 2018 ; Vol. 4, No. 1.
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