A novel mutant gene for (1-3, 1-4)-β-D-glucanless grain on barley (Hordeum vulgare L.) chromosome 7H

Takuji Tonooka, Emiko Aoki, Toji Yoshioka, Shin Taketa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

(1-3, 1-4)-β-D-glucan contained in barley (Hordeum vulgare L.) grains is a main component of endosperm cell walls and constitutes the cell wall matrix with arabinoxylan. A monofactorial recessive mutant gene for (1-3, 1-4)-β-D-glucanless grain was found and the new gene was designated as bgl (= (1-3, 1-4)-beta-Dglucanless grain). A linkage was found between the bgl gene and the naked caryopsis (nud) gene, and it was mapped to the centromeric region of chromosome 7H. Phenotypes in bgl cosegregated with the polymorphisms in HvCslF6, a member of cellulose synthase-like HvCslF gene family, indicating that bgl was caused by a mutation in the HvCslF6 locus. In order to clarify the characteristics of (1-3, 1-4)-β-D-glucanless barley grains, a near-isogenic line (NIL) was developed by backcrossing the Japanese two-rowed cultivar 'Nishinohoshi' as a recurrent parent. The NIL with bgl completely lacked (1-3, 1-4)-β-D-glucan in both the endosperm and aleurone layer cell walls. Microscopic analysis revealed that the NIL had thin endosperm cell walls. It also showed a softer grain texture and many more broken grains during the pearling process than the recurrent parent. The soft and friable grain texture of the NIL was probably caused by the thin endosperm cell walls.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalBreeding Science
Volume59
Issue number1
DOIs
Publication statusPublished - Mar 10 2009

Fingerprint

Hordeum
Endosperm
Cell Wall
Hordeum vulgare
Chromosomes
barley
chromosomes
mutants
isogenic lines
cell walls
endosperm
Genes
genes
glucans
Recessive Genes
Inbreeding
texture
cellulose synthase
aleurone layer
arabinoxylan

Keywords

  • (1-3, 1-4)-β-D-glucanless
  • Barley
  • Cell wall
  • Endosperm
  • Grain hardness
  • Mapping

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

A novel mutant gene for (1-3, 1-4)-β-D-glucanless grain on barley (Hordeum vulgare L.) chromosome 7H. / Tonooka, Takuji; Aoki, Emiko; Yoshioka, Toji; Taketa, Shin.

In: Breeding Science, Vol. 59, No. 1, 10.03.2009, p. 47-54.

Research output: Contribution to journalArticle

Tonooka, Takuji ; Aoki, Emiko ; Yoshioka, Toji ; Taketa, Shin. / A novel mutant gene for (1-3, 1-4)-β-D-glucanless grain on barley (Hordeum vulgare L.) chromosome 7H. In: Breeding Science. 2009 ; Vol. 59, No. 1. pp. 47-54.
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